Circulating Cell-Free DNA Yield and Circulating-Tumor DNA Quantity from Liquid Biopsies of 12 139 Cancer Patients
| Autor: | Eric Allan Severson, Ole Gjoerup, Jeffrey M. Venstrom, Lucas Dennis, Douglas I. Lin, Daniel Duncan, Lei Yang, Jonathan Keith Killian, Jeffrey S. Ross, Shakti H. Ramkissoon, Dean Pavlick, Geoff Oxnard, Richard S.P. Huang, Julia A. Elvin, Jinpeng Xiao, Bernard Fendler, Matthew Hiemenz, Cui Guo, Aparna Aiyer, Dexter X. Jin |
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| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
business.industry Biochemistry (medical) Clinical Biochemistry Liquid Biopsy Urology Cancer medicine.disease Peripheral blood Circulating Cell-Free DNA Circulating Tumor DNA Patient age Circulating tumor DNA Neoplasms Mutation Biomarkers Tumor Humans Medicine Tumor type Stage (cooking) Liquid biopsy business Cell-Free Nucleic Acids Retrospective Studies |
| Zdroj: | Clinical Chemistry. 67:1554-1566 |
| ISSN: | 1530-8561 0009-9147 |
| Popis: | Background The amounts of circulating cell-free DNA (cfDNA) and circulating-tumor DNA (ctDNA) present in peripheral blood liquid biopsies can vary due to preanalytic/analytic variables. In this study, we examined the impact of patient age, sex, stage, and tumor type on cfDNA yield, ctDNA fraction, and estimated ctDNA quantity from a large cohort of clinical liquid biopsy samples. Methods We performed a retrospective analysis of 12 139 consecutive samples received for liquid biopsy (FoundationOne® Liquid) clinical testing. Results Significant differences in both cfDNA yield and estimated ctDNA quantity were observed based on the underlying tumor type that initiated the liquid biopsy analysis and the stage of the patient (P Conclusions In this study, we show that ctDNA quantity varied significantly based on patient age, sex, stage, and tumor type, which could offer an explanation as to why certain liquid biopsy specimens are more likely to fail sequencing or provide clinically meaningful results. In addition, this could affect future clinical decisions on the blood sample volumes required to allow successful liquid biopsy testing. |
| Databáze: | OpenAIRE |
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