Epstein–Barr virus load in transplant patients: Early detection of post-transplant lymphoproliferative disorders
| Autor: | Andrea Bosaleh, Verónica Solernou, María Dolores Fellner, Lucía Irazu, María T. G. de Dávila, Karina Durand, Lidia Virginia Alonio, María Alejandra Picconi, Marcelo Rodriguez, Ziomara Balbarrey |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Epstein-Barr Virus Infections Herpesvirus 4 Human Lymphoma 030230 surgery medicine.disease_cause Epstein–Barr virus Postoperative Complications 0302 clinical medicine hemic and lymphatic diseases Medicine Child PCR en tiempo real Early Detection of Cancer General Medicine Viral Load Virus Epstein-Barr surgical procedures operative Real-time polymerase chain reaction Linfoma Child Preschool Transplant patient Viral load Microbiology (medical) Lymphoid Tissue 030106 microbiology Lymphoproliferative disorders Real-Time Polymerase Chain Reaction Peripheral blood mononuclear cell Microbiology Early PTLD detection Virus Detección temprana de PTLD Immunocompromised Host 03 medical and health sciences Humans Viremia Pacientes trasplantados Retrospective Studies business.industry Infant medicine.disease Kidney Transplantation Lymphoproliferative Disorders Liver Transplantation Carga viral Cross-Sectional Studies DNA Viral Immunology Leukocytes Mononuclear Heart Transplantation Transplant patients business Real-time PCR Follow-Up Studies |
| Zdroj: | Revista Argentina de Microbiología. 48(2):110-118 |
| ISSN: | 0325-7541 |
| DOI: | 10.1016/j.ram.2016.02.006 |
| Popis: | High levels of circulating EBV load are used as a marker of post-transplant lymphoproliferative disorders (PTLD). There is no consensus regarding the threshold level indicative of an increase in peripheral EBV DNA. The aim of the study was to clinically validate a developed EBV quantification assay for early PTLD detection.Transversal study: paired peripheral blood mononuclear cells (PBMC), plasma and oropharyngeal lymphoid tissue (OLT) from children undergoing a solid organ transplant with (n=58) and without (n=47) PTLD. Retrospective follow-up: 71 paired PBMC and plasma from recipients with (n=6) and without (n=6) PTLD history. EBV load was determined by real-time PCR. The diagnostic ability to detect all PTLD (categories 1–4), advanced PTLD (categories 2–4) or neoplastic PTLD (categories 3 and 4) was estimated by analyzing the test performance at different cut-off values or with a load variation greater than 0.5log units.The higher diagnostic performance for identifying all, advanced or neoplastic PTLD, was achieved with cut-off values of 1.08; 1.60 and 2.47log EBVgEq/105 PBMC or 2.30; 2.60; 4.47loggEq/105 OLT cells, respectively. EBV DNA detection in plasma showed high specificity but low (all categories) or high (advanced/neoplastic categories) sensitivity for PTLD identification. Diagnostic performance was greater when: (1) a load variation in PBMC or plasma was identified; (2) combining the measure of EBV load in PBMC and plasma.The best diagnostic ability to identify early PTLD stages was achieved by monitoring EBV load in PBMC and plasma simultaneously; an algorithm was proposed. |
| Databáze: | OpenAIRE |
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