Cell Type-Specific Gene Expression Profiling in Plants by Using a Combination of Laser Microdissection and High-Throughput Technologies
| Autor: | Kazuhiro Ohtsu, Patrick S. Schnable, Hirokazu Takahashi, Mikio Nakazono |
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| Rok vydání: | 2006 |
| Předmět: |
Cell type
Transcription Genetic Microarray Physiology Gene Expression Profiling Lasers Cell Cell Biology Plant Science General Medicine Computational biology Plants Biology Molecular biology Gene expression profiling medicine.anatomical_structure Plant Cells Gene expression medicine Serial analysis of gene expression DNA microarray Microdissection Laser capture microdissection |
| Zdroj: | Plant and Cell Physiology. 48:3-7 |
| ISSN: | 1471-9053 0032-0781 |
| Popis: | Laser microdissection (LM) allows for the isolation of specific cells of interest from heterogeneous tissues under direct microscopic visualization with the assistance of a laser beam. By permitting global analyses of gene expression and metabolites in the selected cells, it is a powerful tool for understanding the biological processes in individual cell types during development or in response to various stimuli. Recently, LM technology has been successfully applied to the separation of individual plant cell types. Here, we provide an overview of applications of LM combined with high-throughput technologies including transcript analyses [microarrays, serial analysis of gene expression (SAGE) and 454-sequencing], proteomic analyses and metabolomic profiling, for cell type-specific gene expression analyses in plants. |
| Databáze: | OpenAIRE |
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