The protein expression landscape of the Arabidopsis root
| Autor: | Molly Megraw, Uwe Ohler, Erik J. Soderblom, Jalean J. Petricka, Stoyan Georgiev, Philip N. Benfey, M.A. Moseley, J. Will Thompson, Ueli Grossniklaus, Monica A. Schauer, Natalie W. Breakfield |
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| Přispěvatelé: | University of Zurich, Benfey, Philip N |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Proteomics
Proteome Cell Arabidopsis Protein Array Analysis Root hair 580 Plants (Botany) Plant Roots Transcriptome 10126 Department of Plant and Microbial Biology Tandem Mass Spectrometry Protein Interaction Mapping medicine 10211 Zurich-Basel Plant Science Center Cellular localization DNA Primers 1000 Multidisciplinary Multidisciplinary Base Sequence biology Arabidopsis Proteins Gene Expression Profiling Biological Sciences Plants Genetically Modified biology.organism_classification Molecular biology Cell biology Gene expression profiling medicine.anatomical_structure RNA Plant Chromatography Liquid |
| DOI: | 10.5167/uzh-74131 |
| Popis: | Because proteins are the major functional components of cells, knowledge of their cellular localization is crucial to gaining an understanding of the biology of multicellular organisms. We have generated a protein expression map of the Arabidopsis root providing the identity and cell type-specific localization of nearly 2,000 proteins. Grouping proteins into functional categories revealed unique cellular functions and identified cell type-specific biomarkers. Cellular colocalization provided support for numerous protein–protein interactions. With a binary comparison, we found that RNA and protein expression profiles are weakly correlated. We then performed peak integration at cell type-specific resolution and found an improved correlation with transcriptome data using continuous values. We performed GeLC-MS/MS (in-gel tryptic digestion followed by liquid chromatography-tandem mass spectrometry) proteomic experiments on mutants with ectopic and no root hairs, providing complementary proteomic data. Finally, among our root hair-specific proteins we identified two unique regulators of root hair development. |
| Databáze: | OpenAIRE |
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