Cultivation-Assisted Genome of Candidatus Fukatsuia symbiotica; the Enigmatic 'X-Type' Symbiont of Aphids

Autor: Vilas Patel, Alejandro Manzano-Marín, Germain Chevignon, Kerry M. Oliver, Jacob A. Russell, Jayce W. Brandt, Michael R. Strand
Přispěvatelé: University of Georgia [USA], Centre de recherche en Biologie Cellulaire (CRBM), Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1), Centre de Biologie pour la Gestion des Populations (UMR CBGP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Drexel University, This work was supported by the US National Science Foundation awards to K.M.O. and M.R.S. (IOS 1256974) and K.M.O. and J.A.R. (IOS 1754302). Also, the Marie-Curie Agreen Skills+ fellowship program cofunded by the European 5 Union's Seventh and a 'Fondation pour la Recherche Medicale' award (ARF201809006914) to G.C. Framework Program (FP7-609938) supported A.M.M., Centre de recherche en Biologie cellulaire de Montpellier (CRBM), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0106 biological sciences
Enterobacteriales
heritable symbiont
bacteria
comparative genomics
insect
evolutionary transition
Hamiltonella defensa
010603 evolutionary biology
01 natural sciences
03 medical and health sciences
Biologie animale
Genetics
Animals
microbiologie
évolution
Symbiosis
Ecology
Evolution
Behavior and Systematics
030304 developmental biology
Animal biology
bactérie
2. Zero hunger
Mutualism (biology)
0303 health sciences
Facultative
Obligate
biology
génome
[SDV.BA]Life Sciences [q-bio]/Animal biology
Microbiology and Parasitology
food and beverages
biochemical phenomena
metabolism
and nutrition
biology.organism_classification
Microbiologie et Parasitologie
Acyrthosiphon pisum
symbiont
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Aphids
aphidae
Candidatus
Buchnera
Gammaproteobacteria
Genome
Bacterial
Research Article
Zdroj: Genome Biology and Evolution
Genome Biology and Evolution, Society for Molecular Biology and Evolution, 2019, 11 (12), pp.3510-3522. ⟨10.1093/gbe/evz252⟩
Genome Biology and Evolution, 2019, 11 (12), pp.3510-3522. ⟨10.1093/gbe/evz252⟩
Genome Biology And Evolution (1759-6653) (Oxford Univ Press), 2019-12, Vol. 11, N. 12, P. 3510-3522
Genome Biology and Evolution 12 (11), 3510-3522. (2019)
ISSN: 1759-6653
DOI: 10.1093/gbe/evz252⟩
Popis: Heritable symbionts are common in terrestrial arthropods and often provide beneficial services to hosts. Unlike obligate, nutritional symbionts that largely persist under strict host control within specialized host cells, heritable facultative symbionts exhibit large variation in within-host lifestyles and services rendered with many retaining the capacity to transition among roles. One enigmatic symbiont, Candidatus Fukatsuia symbiotica, frequently infects aphids with reported roles ranging from pathogen, defensive symbiont, mutualism exploiter and nutritional co-obligate symbiont. Here we used an in vitro culture-assisted protocol to sequence the genome of a facultative strain of Fukatsuia from pea aphids (Acyrthosiphon pisum). Phylogenetic and genomic comparisons indicate that Fukatsuia is an aerobic heterotroph, which together with Regiella insecticola and Hamiltonella defensa form a clade of heritable facultative symbionts within the Yersiniaceae (Enterobacteriales). These three heritable facultative symbionts largely share overlapping inventories of genes associated with housekeeping functions, metabolism, and nutrient acquisition, while varying in complements of mobile DNA. One unusual feature of Fukatsuia is its strong tendency to occur as a co-infection with H. defensa. However, the overall similarity of gene inventories among aphid heritable facultative symbionts suggest that metabolic complementarity is not the basis for co-infection, unless playing out on a H. defensa strain-specific basis. We also compared the pea aphid Fukatsuia with a strain from the aphid Cinara confinis (Lachninae) where it is reported to have transitioned to co-obligate status to support decaying Buchnera function. Overall the two genomes are very similar with no clear genomic signatures consistent with such a transition, which suggests co-obligate status in C. confinis was a recent event.
Databáze: OpenAIRE
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