Theileria parva: Purine and pyrimidine metabolism and the action of folate antagonists in parasitized bovine lymphoid cells
| Autor: | A.D. Irvin, D.A. Stagg |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Purine
Guanine Immunology Purine analogue Biology chemistry.chemical_compound Pyrimidine analogue Animals Lymphocytes Purine metabolism Uridine Cells Cultured Hypoxanthine Adenine Azaguanine Eukaryota General Medicine Pyrimidine Nucleosides Molecular biology Aminopterin Culture Media Theileriasis Methotrexate Infectious Diseases Biochemistry chemistry Purines Hypoxanthine-guanine phosphoribosyltransferase Hypoxanthines Pyrimidine metabolism Folic Acid Antagonists Cattle Parasitology Floxuridine Thymidine |
| Zdroj: | Experimental Parasitology. 41:172-185 |
| ISSN: | 0014-4894 |
| DOI: | 10.1016/0014-4894(77)90143-6 |
| Popis: | The in vitro growth of bovine lymphoid cells infected with Theileria parva was inhibited by the folate antagonists aminopterin and methotrexate, even in the presence of exogenous hypoxanthine and thymidine. Further studies using tritiated purines and pyrimidines, showed that adenine and thymidine were incorporated into cells and macroschizonts and that uridine was incorporated at a similar level but without evidence of specific localization. There was a generalized low level of incorporation of hypoxanthine into cells but no incorporation of guanine. It was also shown that the pyrimidine analog, fluorodeoxyuridine, was readily incorporated by cells and macroschizonts, but that the purine analog, 8-azaguanine, was not readily incorporated by the cells and not at all by the macroschizonts. The combined results indicated that there was a deficiency, or reduction in activity, of the enzyme hypoxanthine guanine phosphoribosyl transferase (HGPRT, EC 2.4.2.8) preventing purine metabolism by a salvage pathway when the main purine pathway was blocked. HGPRT deficiency appeared to be partial in the cells but complete in the macroschizonts. No defect in pyrimidine metabolism by cells or macroschizonts was detected. Because of the apparent defect in purine metabolism of T. parva-infected cells, it is suggested that folate antagonists may have application in the therapy of East Coast fever. |
| Databáze: | OpenAIRE |
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