Potentiated autologous tumor cell and peripheral blood lymphocyte lysate vaccination
| Autor: | Sebastiano Rizzo, Maria Grazia Silvotti |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
medicine.drug_class
Lymphocyte medicine.medical_treatment Biopsy Cell Culture Techniques Bioengineering Monoclonal antibody Applied Microbiology and Biotechnology Biochemistry Cancer Vaccines Neoplasms medicine Tumor Cells Cultured Humans Leukapheresis Lymphocytes Molecular Biology Cells Cultured biology business.industry Immunotherapy Active Immunotherapy Autologous tumor cell Coculture Techniques medicine.anatomical_structure Cell culture Peripheral blood lymphocyte Immunology biology.protein Interleukin-2 Cancer vaccine Antibody business Biotechnology |
| Zdroj: | Molecular biotechnology. 25(1) |
| ISSN: | 1073-6085 |
| Popis: | Immune stimulation is a promising prospect in cancer therapy. Immunotherapy may be local or systemic, aspecific or targeted and may use monoclonal antibodies or vaccines. The aim of using vaccines is to stimulate the body to produce its own antibodies. Autologous tumor-cell vaccination has no contraindications or side-effects, since the patients own materials (lymphocytes, tumor cells) are used. We describe a method for producing an autologous cancer vaccine. The material to be injected as a vaccine derives from a mixed culture of autologous lymphocytes cocultured with autologous cancer cells. Peripheral blood lymphocytes are obtained by lymphocytapheresis. Cancer cells may be obtained from tissue biopsies or biological fluids, or from long-term cultures from the patient who is to be vaccinated. The culture medium (RPMI 1640) is free of fetal calf serum (FCS). The coculture is mixed with autologous plasma in a 1:1 ratio with the addition of 200 IU of recombinant human interleukin-2/mL, and is incubated at 37 degrees C in a humidified 5% CO2-enriched atmosphere for 48 h. The cocultured material is frozen, thawed to lyse cells, aliquoted and stored at -20 degrees C. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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