Detection by PCR of pathogenic protozoa in raw and drinkable water samples in Colombia
Autor: | Juan David Zuluaga, Fabiana Lora, Jorge Enrique Gómez-Marín, Jessica Triviño-Valencia |
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Rok vydání: | 2016 |
Předmět: |
Giardiasis
0301 basic medicine animal diseases 030231 tropical medicine 030106 microbiology Protozoan Proteins Cryptosporidiosis Colombia Biology medicine.disease_cause Polymerase Chain Reaction Water Purification Microbiology law.invention Feces 03 medical and health sciences 0302 clinical medicine law parasitic diseases medicine Animals Humans Giardia lamblia Phylogeny Polymerase chain reaction Cryptosporidium parvum Base Sequence General Veterinary Drinking Water Toxoplasma gondii Giardia Cryptosporidium General Medicine DNA Protozoan biology.organism_classification Fecal coliform Infectious Diseases Fluorescent Antibody Technique Direct Insect Science Parasitology Toxoplasma Cryptosporidium hominis Toxoplasmosis |
Zdroj: | Parasitology Research. 115:1789-1797 |
ISSN: | 1432-1955 0932-0113 |
Popis: | We evaluated the presence of DNA of Giardia, Toxoplasma, and Cryptosporidium by PCR, and of Giardia and Cryptosporidium genera by immunofluorescence antibody test (IFAT), in water samples, before, during, and after plant treatment for drinkable water. We applied this method in 38 samples of 10 l of water taken from each of the water treatment steps and in 8 samples taken at home (only for Toxoplasma PCR) in Quindio region in Colombia. There were 8 positive samples for Cryptosporidium parvum (21 %), 4 for Cryptosporidium hominis (10.5 %), 27 for Toxoplasma gondii (58.6 %), 2 for Giardia duodenalis assemblage A (5.2 %), and 5 for G. duodenalis assemblage B (13.1 %). By IFAT, 23 % were positive for Giardia and 21 % for Cryptosporidium. An almost perfect agreement was found between IFAT and combined results of PCR, by Kappa composite proportion analysis. PCR positive samples were significantly more frequent in untreated raw water for C. parvum (p = 0.02). High mean of fecal coliforms, high pH values, and low mean of chlorine residuals were strongly correlated with PCR positivity for G. duodenalis assemblage B. High pH value was correlated with PCR positivity for C. parvum. Phylogenetic analysis of DNA sequences was possible, showing water and human clinical sequences for Toxoplasma within the same phylogenetic group for B1 repeated sequence. PCR assay is complementary to IFAT assay for monitoring of protozoa in raw and drinkable water, enabling species identification and to look for phylogenetic analysis in protozoa from human and environmental sources. |
Databáze: | OpenAIRE |
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