Establishing a rabbit model of malignant esophagostenosis using the endoscopic implantation technique for studies on stent innovation
| Autor: | Xiang Wang, Zhi Liu, Hanming Song, Guanyin Xiong, Xiaowei Tang, Yin Zhang, Yingzhou Shen, Zhining Fan, Jin Huang, Jinquan Shuang |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Prosthesis Implantation Endoscopic surgical procedure Rabbit Kaplan-Meier Estimate General Biochemistry Genetics and Molecular Biology Esophagus Stent therapy Esophageal squamous cell carcinoma medicine Animals Humans In patient Animal model cardiovascular diseases Medicine(all) medicine.diagnostic_test business.industry Biochemistry Genetics and Molecular Biology(all) Methodology Stent Endoscopy General Medicine medicine.disease equipment and supplies Dysphagia Surgery Disease Models Animal surgical procedures operative Esophageal stricture Rabbit model Esophageal Stenosis Female Stents Rabbits medicine.symptom business Esophagostenosis |
| Zdroj: | Journal of Translational Medicine |
| ISSN: | 1479-5876 |
| Popis: | Background Stents are recommended in patients with dysphagia caused by esophageal stricture, but an ideal stent does not currently exist. Thus, studies on new esophageal stents are necessary, and suitable animal models are desperately needed for these studies. The aim of this study was to establish a model of malignant esophageal stricture in rabbit for studies on stent innovation. Methods A total of 38 New Zealand white rabbits were used in this study. Using the endoscopic submucosal injection technique, VX2 fragments were inoculated into the submucosal layer of the rabbit thoracic esophagus, and an endoscopic follow-up was subsequently performed to observe the tumor development and progression. The self-expandable metal stents were randomly deployed in rabbits with severe esophageal stricture to investigate the safety and feasibility of the animal models for stenting. Results An endoscopic implantation procedure for VX2 tumors was completed in 34/38 rabbits, and tumor development was confirmed in 30/34 animals. The success rate of the endoscopic implantation and tumor development were 89.4% (95% CI, 79.6% to 99.2%) and 88.2% (95% CI, 76.9% to 99.5%) respectively. During the endoscopic follow-up period, severe esophageal stricture occurred in 22/30 rabbits with a rate of 73.3% (95% CI, 57.5% to 89.1%), and 12/22 models received stent placement. During and after stent implantation, no severe stent-related complication or mortality occurred in the animal models. The rabbits that received stent placement survived longer than those without stent implantation (the mean survival time: 53.9 days versus 40.3 days, P = 0.016). Conclusion The endoscopic method is a safe and effective method for establishing a malignant esophagostenosis model in rabbits. This model can simulate the human body environment for stent deployment and is an excellent tool for the study of stent innovation for the treatment of esophageal cancer. |
| Databáze: | OpenAIRE |
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