Changes in Hyaluronan Deposition during Early Respiratory Distress Syndrome in Premature Monkeys
| Autor: | M G Kinsella, William E. Truog, J. C. Jackson, Thomas A. Standaert, Sandra E. Juul, W. A. Hodson |
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| Rok vydání: | 1994 |
| Předmět: |
medicine.medical_specialty
Pathology medicine.medical_treatment High-Frequency Ventilation Lung injury Pregnancy Fibrosis Internal medicine medicine Animals Humans Hyaluronic Acid Lung Mechanical ventilation Respiratory Distress Syndrome Newborn Respiratory distress business.industry High-frequency ventilation Respiratory disease Infant Newborn Pulmonary Surfactants respiratory system medicine.disease respiratory tract diseases Pulmonary Alveoli Endocrinology medicine.anatomical_structure Animals Newborn Bronchopulmonary dysplasia Pediatrics Perinatology and Child Health Female Macaca nemestrina business |
| Zdroj: | Pediatric Research. 35:238-243 |
| ISSN: | 1530-0447 0031-3998 |
| DOI: | 10.1203/00006450-199402000-00024 |
| Popis: | Increased deposition of hyaluronan (HA) is part of the early response to fibrogenic stimulus in the lung exposed to bleomycin injury and has been associated with increased lung water in adult animals. Early respiratory distress syndrome (RDS) in premature infants is characterized by increased lung water, and late sequelae include fibrosis or bronchopulmonary dysplasia. We hypothesized that increased HA in the alveolar interstitium would be associated with increasingly severe RDS in prematurely delivered monkeys and that modes of therapy that affect severity of disease such as treatment with high-frequency oscillatory ventilation or exogenous surfactant would decrease this response. Thirty-four Macaca nemestrina monkeys were delivered at 134 +/- 1 d (term = 168 d) and randomized to high-frequency oscillatory ventilation or conventional mechanical ventilation from birth. Sixteen of these animals received surfactant. At 6 h of age, the right lower lung was frozen in situ during inflation to 30 cm H2O (approximately 2940 Pa) and then dehydrated and processed for microscopy. The presence and severity of RDS were evaluated by clinical and morphologic criteria. HA concentrations in lung extracts increased with progressively severe RDS (p = 0.0003). Treatment with high-frequency oscillatory ventilation decreased the lung injury score (1.69 +/- 0.7 compared with 2.5 +/- 0.9, p = 0.05), but changes in lung HA concentration did not reach significance (37.9 +/- 22.7 compared with 44.8 +/- 22.6). Surfactant treatment decreased lung HA concentration (29.6 +/- 19.0 micrograms/wet lung) compared with non-surfactant-treated animals (54.7 +/- 20.2 micrograms/g wet lung, p = 0.0009).(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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