Matrix metalloproteinase (MMP)-2, MMP-9, semen quality and sperm longevity in fractionated stallion semen
| Autor: | Maria Kareskoski, Anders Johannisson, Mari Palviainen, Kirsi Laukkanen, Johanna Vakkamäki, Terttu Katila |
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| Přispěvatelé: | Production Animal Medicine, Animal Reproduction Science, Helsinki One Health (HOH), Departments of Faculty of Veterinary Medicine, HUS Inflammation Center, Equine and Small Animal Medicine, Extracellular Vesicles |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
endocrine system media_common.quotation_subject Gelatin Zymography Longevity Semen Biology Matrix metalloproteinase 413 Veterinary science Andrology 03 medical and health sciences Semen quality 0302 clinical medicine Food Animals Animals Horses Ejaculate Small Animals Sperm motility Seminal plasma media_common Stallion 030219 obstetrics & reproductive medicine Equine urogenital system 0402 animal and dairy science 04 agricultural and veterinary sciences 040201 dairy & animal science Sperm Spermatozoa Semen Analysis Matrix Metalloproteinase 9 Sperm longevity Sperm Motility Matrix Metalloproteinase 2 Animal Science and Zoology Semen Preservation |
| Popis: | Matrix metalloproteinase (MMP)-2 and MMP-9 are gelatinases that take part in several reproductive processes. The aim of this study was to measure levels of MMP-2 and MMP-9 in fractionated stallion ejaculates, and to evaluate the association between these components and semen quality, and sperm longevity during cooled storage. Semen quality were assessed separately for sperm-rich fractions (HIGH), sperm-poor fractions (LOW), and whole ejaculate samples (WE) from 33 stallions. After cooled storage with SP either present or removed, sperm motility and DFI were determined. The relative activity of the pro-form of MMP-2, active MMP-2 and total MMP-9 were evaluated using gelatin zymography, and all were present in all fractions of the stallion's ejaculate, with higher relative activity of the latent than active forms and the highest relative activity in the HIGH fraction. The relative activities of MMP-2 and MMP-9 were positively correlated to sperm concentration and total sperm count, but only in the HIGH fraction and not in LOW or WE. The relative activities of MMPs were not related to differences in sperm longevity during cooled storage, measured as sperm motility and DFI. There was a harmful effect of SP on DFI during storage, but this effect was not associated with differences in the relative activities of MMPs. In conclusion, the relative activities of MMPs are not useful as markers for semen quality (other than sperm concentration), or sperm survival during storage in horses. (C) 2021 Elsevier Inc. All rights reserved. |
| Databáze: | OpenAIRE |
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