Development of a novel SNP assay to detect lactase persistence associated genetic variants
| Autor: | Laura de Magistris, Coluccia Enza, Patrizia Iardino, Bruna De Felice, Elio Biffali, Maria Rosaria Amato, Pasquale De Luca, Diego Pappalardo, Concetta Montanino, Daniela Iaconis, Gabriele Riegler |
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| Přispěvatelé: | Pasquale De Luca, Daniela, Iaconi, Elio, Biffali, Coluccia, Enza, Laura de Magistris, Gabriele, Riegler, Diego, Pappalardo, Maria Rosaria Amato, Patrizia, Iardino, Concetta, Montanino, DE FELICE, Bruna |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Persistence (psychology)
Adult Male medicine.medical_treatment Fingerprint SNP Single-nucleotide polymorphism Biology Polymorphism Single Nucleotide Primer extension symbols.namesake Lactose Intolerance Genotype Genetics medicine Humans Molecular Biology Lactase Sanger sequencing General Medicine Middle Aged Lactase persistence symbols Genomic Female Original Article |
| Zdroj: | Molecular Biology Reports |
| Popis: | Background In adulthood the activity of the lactase enzyme is inherited as autosomal dominant form associated to Single nucleotide polymorphisms (SNPs). The present research was aimed to develop a novel genetic method to test lactase non persistence more powerfully. Methods and results In our study, we selected eight different SNPs that are associated with lactase persistence from Caucasian, Arabian Bedouins, sub-Saharian Africans and Asian populations to set up an approach to detect all the eight different SNPs at the same time in the same sample. This technique is centred on the identification of SNPs with a single nucleotide primer extension method using Sanger sequencing and capillary electrophoresis. Conclusions Our method allowed us to check the genotype asset of eight SNPs related to lactase persistence simultaneously and in a very efficient manner. It could be applied to a higher number of SNPs in a single reaction. |
| Databáze: | OpenAIRE |
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