Rapid identification of strains belonging to the Mycobacterium abscessus group through erm(41) gene pyrosequencing
| Autor: | Katsuhiro Suzuki, Ryoko Shimada, Motohisa Tomita, Seiji Hayashi, Masaji Okada, Kazunari Tsuyuguchi, Shiomi Yoshida |
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| Rok vydání: | 2014 |
| Předmět: |
DNA
Bacterial Microbiology (medical) Molecular Sequence Data Mycobacterium abscessus DNA Ribosomal Polymorphism Single Nucleotide Mycobacterium Microbiology Bacterial Proteins RNA Ribosomal 16S Mycobacterium bolletii DNA Ribosomal Spacer Humans Gene Bacteriological Techniques tRNA Methyltransferases Mycobacterium massiliense Base Sequence biology Chaperonin 60 DNA-Directed RNA Polymerases Sequence Analysis DNA General Medicine biochemical phenomena metabolism and nutrition bacterial infections and mycoses 16S ribosomal RNA biology.organism_classification rpoB Rapid identification RNA Ribosomal 23S Infectious Diseases Molecular Diagnostic Techniques bacteria Pyrosequencing |
| Zdroj: | Diagnostic Microbiology and Infectious Disease. 79:331-336 |
| ISSN: | 0732-8893 |
| DOI: | 10.1016/j.diagmicrobio.2014.04.001 |
| Popis: | Mycobacterium abscessus and Mycobacterium massiliense lung infections have different clarithromycin susceptibilities, making proper identification important; however, standard multi-gene sequencing in clinical laboratories is laborious and time consuming. We developed a pyrosequencing-based method for rapid identification of strains belonging to the M. abscessus group by targeting erm(41). We examined 55 isolates from new pulmonary M. abscessus infections and identified 28 M. abscessus, 25 M. massiliense, and 2 Mycobacterium bolletii isolates. Multi-gene sequencing of 16S rRNA, hsp65, rpoB, and the 16S-23S ITS region was concordant with the results of erm(41) pyrosequencing; thus, the M. abscessus group can be identified by single-nucleotide polymorphisms in erm(41). The method also enables rapid identification of polymorphic, inducible clarithromycin-resistant sequevars (T28 or C28). Pyrosequencing of erm(41) is a rapid, reliable, high-throughput alternative method for identifying and characterizing M. abscessus species. Further testing of a diverse collection of isolates is necessary to demonstrate the discriminatory power of erm(41) sequencing to differentiating species with this highly divergent group. |
| Databáze: | OpenAIRE |
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