Potential role of Candida albicans secreted aspartic protease 9 in serum induced-hyphal formation and interaction with oral epithelial cells
| Autor: | Otto L.T. Lam, Haiping Yang, Paul Wai Kei Tsang, Edmond H.N. Pow, Peter C.S. Tsang |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Hypha 030106 microbiology Hyphae Endocytosis Microbiology Cell Line Agar plate Fungal Proteins 03 medical and health sciences Candidiasis Oral Gene Expression Regulation Fungal Candida albicans Aspartic Acid Endopeptidases biology Chemistry Kinase fungi Mouth Mucosa biology.organism_classification Molecular biology Corpus albicans Yeast 030104 developmental biology Infectious Diseases Host-Pathogen Interactions Mutation Fetal bovine serum |
| Zdroj: | Microbial pathogenesis. 139 |
| ISSN: | 1096-1208 |
| Popis: | Introduction Candida albicans possesses the ability to switch rapidly between yeast to hyphal forms. Hyphal formation is a remarkable pathogenic characteristic, which allows C. albicans to invade into host cells. Objectives This study was to investigate the role of the C. albicans SAP9 gene in hyphal formation and invasion ability. Methods The morphology of fungal cells in the hyphal-inducing liquid media (YPD+10% fetal bovine serum) was observed by the microscopy. And the morphology of the colony on solid agar plates of YPD+10% fetal bovine serum was photographed by the digital camera. The mRNA expressions of hypha-associated genes in serum medium were also analyzed by real time PCR. Then for the interaction between C. albicans and oral epithelial cells, endocytosis essay, invasion essay and damage assay were performed to compare the differences between the sap9Δ/Δ mutant strain and wild type strain. Results Compared with the wild type strain, the sap9Δ/Δ mutant strain exhibited a deficient yeast-to-hyphal morphological transition under serum hyphal-inducing conditions. Furthermore, the SAP9 knockout strain revealed a significant down-regulation of the expression of EFG1 (~40%), which is a transcription factor gene that mediates hyphae formation in C. albicans. Compared with the wild type strain, a 70% reduction in the endocytosis of the sap9Δ/Δ mutant strain by host cells was observed, as well as a 25% attenuation of active penetration and a 40% attenuation of host cell damage (P Conclusions Our data strongly suggests that C. albicans Sap9 is a potential hyphal-associated factor that responds to serum hyphal-inducing stimuli via a cAMP-protein kinase A pathway mediated by EFG1, and contributes to the process of invasion of Candida into the epithelial cells, leading to host cell damage. |
| Databáze: | OpenAIRE |
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