Porphyromonas gingivalis infection-induced tissue and bone transcriptional profiles
| Autor: | Vasudevan Bakthavatchalu, Shannon M. Wallet, J. L. Ebersole, Maria-Cecilia Lopez, Martin Handfield, Sabapathi Sathishkumar, Brendan F. Boyce, Richard J. Lamont, Indraneel Bhattacharyya, A. Meka, Lakshmyya Kesavalu, Raj K. Verma, Henry V. Baker |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Microbiology (medical)
Pathology medicine.medical_specialty Transcription Genetic medicine.medical_treatment Immunology Osteoclasts Calvaria Inflammation Microbiology Bone resorption Article Bone and Bones Proinflammatory cytokine Mice Immune system medicine Bacteroidaceae Infections Animals Bone Resorption General Dentistry Porphyromonas gingivalis Oligonucleotide Array Sequence Analysis Mice Inbred BALB C biology Gene Expression Profiling Toll-Like Receptors Soft tissue biology.organism_classification Molecular biology medicine.anatomical_structure Cytokine Host-Pathogen Interactions Cytokines Female medicine.symptom Inflammation Mediators Cell Adhesion Molecules |
| Popis: | Porphyromonas gingivalis has been associated with subgingival biofilms in adult periodontitis. However, the molecular mechanisms of its contribution to chronic gingival inflammation and loss of periodontal structural integrity remain unclear. This investigation aimed to examine changes in the host transcriptional profiles during a P. gingivalis infection using a murine calvarial model of inflammation and bone resorption. P. gingivalis FDC 381 was injected into the subcutaneous soft tissue over the calvaria of BALB/c mice for 3 days, after which the soft tissues and calvarial bones were excised. RNA was isolated from infected soft tissues and calvarial bones and was analysed for transcript profiles using Murine GeneChip((R)) arrays to provide a molecular profile of the events that occur following infection of these tissues. After P. gingivalis infection, 6452 and 2341 probe sets in the infected soft tissues and calvarial bone, respectively, were differentially expressed (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|