Polyamine metabolism of rat gastric mucosa after oral administration of hypertonic sodium chloride solution
| Autor: | Tadayoshi Hasuma, Kenjiro Otani, Tetsuo Arakawa, Yoshihisa Yano, Kenzo Kobayashi, Isao Matsui-Yuasa, Shuzo Otani |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
DNA Replication
Male medicine.medical_specialty Eflornithine Physiology Administration Oral Ornithine Decarboxylase Ornithine decarboxylase chemistry.chemical_compound Acetyltransferases Oral administration Physiology (medical) Internal medicine Polyamines Putrescine medicine Gastric mucosa Animals Enzyme Inhibitors Rats Wistar Saline Solution Hypertonic Oxidoreductases Acting on CH-NH Group Donors Hepatology Stomach Gastroenterology Metabolism Rats Spermidine medicine.anatomical_structure Endocrinology chemistry Gastric Mucosa Polyamine |
| Zdroj: | American Journal of Physiology-Gastrointestinal and Liver Physiology. 274:G299-G305 |
| ISSN: | 1522-1547 0193-1857 |
| DOI: | 10.1152/ajpgi.1998.274.2.g299 |
| Popis: | Oral administration of 1 ml of 3.42 M NaCl solution to rats induced spermidine/spermine N1-acetyltransferase (SSAT) activity in gastric mucosa as well as ornithine decarboxylase (ODC) activity. SSAT activity increased and peaked at 5 h and again at 7 h, whereas ODC activity peaked at 6 h. SSAT mRNA also increased after 3.42 M NaCl administration to an extent similar to the increase in SSAT activity at 5 h. Intracellular putrescine level and DNA synthesis were increased by NaCl administration. A polyamine oxidase inhibitor, N, N′-bis(2,3-butadienyl)-1,4-butanediamine (MDL-72527), but not an ODC inhibitor, α-difluoromethylornithine (DFMO), inhibited the increases in putrescine level and DNA synthesis at 5 h. The inhibition of DNA synthesis by MDL-72527 was reversed by putrescine administration. In contrast, both MDL-72527 and DFMO inhibited the increase in putrescine level and DNA synthesis at 16.5 h. These findings suggest that putrescine produced from preexistent spermidine by SSAT is responsible for the initial DNA synthesis after mucosal injury induced by NaCl and that both SSAT and ODC are involved in formation of putrescine, which is required for subsequent DNA synthesis. |
| Databáze: | OpenAIRE |
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