Innovative Biocatalysts as Tools to Detect and Inactivate Nerve Agents
| Autor: | Valentina De Luca, Piero Porcaro, Elena Porzio, Franz Worek, Ilaria Palchetti, Antonio Pisanti, Giuseppe Manco, Teresa Maria Carusone, Immacolata Del Giudice, Mario De Rosa, Odile F. Restaino, Maria Giovanna Borzacchiello, Chiara Schiraldi, Serena Laschi, Francesca Bettazzi, Luigi Mandrich, Ferdinando Febbraio |
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| Přispěvatelé: | Porzio, E., F., Bettazzi, L., Mandrich, I., Del Giudice, Restaino, O. F., Laschi, S., Febbraio, F., De Luca, V., Borzacchiello, M. G., Carusone, T. M., Worek, F., Pisanti, A., Porcaro, P., Schiraldi, C., De Rosa, M., Palchetti, I., Manco, G. |
| Jazyk: | Creoles and pidgins, English based |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Sulfolobus acidocaldarius Alicyclobacillus ved/biology.organism_classification_rank.species lcsh:Medicine 02 engineering and technology Biosensing Techniques nerve agents Article 03 medical and health sciences Environmental Biotechnology Organophosphorus Compounds organophosphates Limit of Detection Detoxification medicine Electrochemical biosensor Chemical Warfare Agents Pesticides lcsh:Science Decontamination Nerve agent Multidisciplinary Chemistry ved/biology Biosensing assay systems Sulfolobus solfataricus lcsh:R Human decontamination 021001 nanoscience & nanotechnology Combinatorial chemistry Acute toxicity 030104 developmental biology Biosensors Phosphoric Triester Hydrolases Biocatalysis lcsh:Q 0210 nano-technology Alicyclobacillus acidocaldarius Biomaterials-protein Carboxylic Ester Hydrolases medicine.drug |
| Zdroj: | 13th International Meeting on Cholinesterases-7th International Conference on Paraoxonases", 9-14/9/2018. info:cnr-pdr/source/autori:Elena Porzio1*, Francesca Bettazzi2*, Luigi Mandrich1, Immacolata Del Giudice1, Odile F. Restaino3, Serena Laschi4, Ferdinando Febbraio1, Valentina De Luca1, Maria G. Borzacchiello3, Teresa M. Carusone1, Franz Worek5, Antonio Pisanti6, Piero Porcaro6, Chiara Schiraldi3, Mario De Rosa3, Ilaria Palchetti2, Giuseppe Manco1#/congresso_nome:13th International Meeting on Cholinesterases-7th International Conference on Paraoxonases"/congresso_luogo:/congresso_data:9-14%2F9%2F2018./anno:2018/pagina_da:/pagina_a:/intervallo_pagine Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-13 (2018) Scientific reports (Nature Publishing Group) 8 (2018). doi:10.1038/s41598-018-31751-5 info:cnr-pdr/source/autori:Porzio, Elena; Bettazzi, Francesca; Mandrich, Luigi; Del Giudice, Immacolata; Restaino, Odile F.; Laschi, Serena; Febbraio, Ferdinando; De Luca, Valentina; Borzacchiello, Maria G.; Carusone, Teresa M.; Worek, Franz; Pisanti, Antonio; Porcaro, Piero; Schiraldi, Chiara; De Rosa, Mario; Palchetti, Ilaria; Manco, Giuseppe/titolo:Innovative Biocatalysts as Tools to Detect and Inactivate Nerve Agents/doi:10.1038%2Fs41598-018-31751-5/rivista:Scientific reports (Nature Publishing Group)/anno:2018/pagina_da:/pagina_a:/intervallo_pagine:/volume:8 |
| DOI: | 10.1038/s41598-018-31751-5 |
| Popis: | Pesticides and warfare nerve agents are frequently organophosphates (OPs) or related compounds. Their acute toxicity highlighted more than ever the need to explore applicable strategies for the sensing, decontamination and/or detoxification of these compounds. Herein, we report the use of two different thermostable enzyme families capable to detect and inactivate OPs. In particular, mutants of carboxylesterase-2 from Alicyclobacillus acidocaldarius and of phosphotriesterase-like lactonases from Sulfolobus solfataricus and Sulfolobus acidocaldarius, have been selected and assembled in an optimized format for the development of an electrochemical biosensor and a decontamination formulation, respectively. The features of the developed tools have been tested in an ad-hoc fabricated chamber, to mimic an alarming situation of exposure to a nerve agent. Choosing ethyl-paraoxon as nerve agent simulant, a limit of detection (LOD) of 0.4 nM, after 5 s of exposure time was obtained. Furthermore, an optimized enzymatic formulation was used for a fast and efficient environmental detoxification (>99%) of the nebulized nerve agent simulants in the air and on surfaces. Crucial, large-scale experiments have been possible thanks to production of grams amounts of pure (>90%) enzymes. |
| Databáze: | OpenAIRE |
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