Preparation of acellular scaffold for corneal tissue engineering by supercritical carbon dioxide extraction technology
| Autor: | Shu Wei Wu, Dar Jen Hsieh, Wen Hsin Chang, Yi Hsun Huang, Fan Wei Tseng, I. Chen Peng, Ming Long Yeh |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Scaffold Materials science Lysis Biocompatibility Swine medicine.medical_treatment Biomedical Engineering 02 engineering and technology Biochemistry Biomaterials Cornea Corneal Transplantation 03 medical and health sciences Tissue engineering medicine Animals Humans Molecular Biology Corneal transplantation Decellularization Tissue Engineering Tissue Scaffolds Extraction (chemistry) General Medicine Carbon Dioxide 021001 nanoscience & nanotechnology eye diseases 030104 developmental biology medicine.anatomical_structure Heterografts sense organs Rabbits 0210 nano-technology Biotechnology Biomedical engineering |
| Zdroj: | Acta biomaterialia. 58 |
| ISSN: | 1878-7568 |
| Popis: | In this study, we developed a novel method using supercritical carbon dioxide (SCCO 2 ) to prepare acellular porcine cornea (APC). Under gentle extraction conditions using SCCO 2 technology, hematoxylin and eosin staining showed that cells were completely lysed, and cell debris, including nuclei, was efficiently removed from the porcine cornea. The SCCO 2 -treated corneas exhibited intact stromal structures and appropriate mechanical properties. Moreover, no immunological reactions and neovascularization were observed after lamellar keratoplasty in rabbits. All transplanted grafts and animals survived without complications. The transplanted APCs were opaque after the operation but became transparent within 2 weeks. Complete re-epithelialization of the transplanted APCs was observed within 4 weeks. In conclusion, APCs produced by SCCO 2 extraction technology could be an ideal and useful scaffold for corneal tissue engineering. Statement of significance We decellularized the porcine cornea using SCCO 2 extraction technology and investigated the characteristics, mechanical properties, and biocompatibility of the decellularized porcine cornea by lamellar keratoplasty in rabbits. To the best of our knowledge, this is the first report describing the use of SCCO 2 extraction technology for preparation of acellular corneal scaffold. We proved that the cellular components of porcine corneas had been efficiently removed, and the biomechanical properties of the scaffold were well preserved by SCCO 2 extraction technology. SCCO 2 -treated corneas maintained optical transparency and exhibited appropriate strength to withstand surgical procedures. In vivo, the transplanted corneas showed no evidence of immunological reactions and exhibited good biocompatibility and long-term stability. Our results suggested that the APCs developed by SCCO 2 extraction technology could be an ideal and useful scaffold for corneal replacement and corneal tissue engineering. |
| Databáze: | OpenAIRE |
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