Decreased Fast Ripples in the Hippocampus of Rats with Spontaneous Recurrent Seizures Treated with Carbenoxolone and Quinine
| Autor: | Laura Medina-Ceja, Consuelo Ventura-Mejía |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty animal structures Article Subject Carbenoxolone lcsh:Medicine Hippocampus Epileptogenesis General Biochemistry Genetics and Molecular Biology Temporal lobe Epilepsy Recurrence Seizures Internal medicine medicine Animals Rats Wistar Behavior Animal Quinine General Immunology and Microbiology business.industry lcsh:R Gap junction Electroencephalography General Medicine Entorhinal cortex medicine.disease Endocrinology Pilocarpine business Research Article medicine.drug |
| Zdroj: | BioMed Research International, Vol 2014 (2014) BioMed Research International |
| ISSN: | 2314-6141 2314-6133 |
| DOI: | 10.1155/2014/282490 |
| Popis: | Background. In models of temporal lobe epilepsy and in patients with this pathology, high frequency oscillations called fast ripples (FRs, 250–600 Hz) can be observed. FRs are considered potential biomarkers for epilepsy and, in the light of manyin vitroandin silicostudies, we thought that electrical synapses mediated by gap junctions might possibly modulate FRsin vivo.Methods. Animals with spontaneous recurrent seizures induced by pilocarpine administration were implanted with movable microelectrodes in the right anterior and posterior hippocampus to evaluate the effects of gap junction blockers administered in the entorhinal cortex. The effects of carbenoxolone (50 nmoles) and quinine (35 pmoles) on the mean number of spontaneous FR events (occurrence of FRs), as well as on the mean number of oscillation cycles per FR event and their frequency, were assessed using a specific algorithm to analyze FRs in intracranial EEG recordings.Results. We found that these gap junction blockers decreased the mean number of FRs and the mean number of oscillation cycles per FR event in the hippocampus, both during and at different times after carbenoxolone and quinine administration.Conclusion. These data suggest that FRs may be modulated by gap junctions, although additional experimentsin vivowill be necessary to determine the precise role of gap junctions in this pathological activity associated with epileptogenesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|