Partial purification and characterization of acetylcholinesterase (AChE) from the estuarine copepod Eurytemora affinis (Poppe)
| Autor: | Cailleaud, K., Forget-Leray, J., Souissi, S., Lardy, S., Augagneur, S., Budzinski, H., Forget, Joëlle, Livet, Sandrine, Leboulenger, François |
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| Přispěvatelé: | Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS) |
| Rok vydání: | 2002 |
| Předmět: |
Physiology
Health Toxicology and Mutagenesis 010501 environmental sciences Toxicology 01 natural sciences Biochemistry Substrate Specificity Butyrylthiocholine Copepoda 03 medical and health sciences chemistry.chemical_compound Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Isoelectric Point ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0105 earth and related environmental sciences Cholinesterase chemistry.chemical_classification 0303 health sciences Chromatography integumentary system biology Isoelectric focusing Cell Biology General Medicine biology.organism_classification Acetylcholinesterase Crustacean Molecular Weight Kinetics Enzyme chemistry 13. Climate action biology.protein Cholinesterase Inhibitors Isoelectric Focusing Bioindicator Copepod |
| Zdroj: | Comparative Biochemistry and Physiology-Part C: Toxicology and Pharmacology Comparative Biochemistry and Physiology-Part C: Toxicology and Pharmacology, Elsevier, 2002, 132 (1), pp.85-92. ⟨10.1016/S1532-0456(02)00050-9⟩ |
| ISSN: | 1532-0456 |
| DOI: | 10.1016/s1532-0456(02)00050-9 |
| Popis: | Oligohaline copepods such as Eurytemora affinis are widespread in estuaries of northwestern Europe. These minute crustaceans are highly sensitive to contamination and thus serve as useful bioindicators for the monitoring of pollutant effects. The use of decreased cholinesterase (ChE) activity as a sublethal biomarker of exposure to neurotoxic compounds supposes that ChE has been defined in copepods. This study reports the partial purification and characterization of ChE extracted from E . affinis. Analysis by non-denaturing PAGE and by isoelectric focusing indicated that the enzyme is probably a single dimeric form of 140 KDa, with a p I of 6.2. This enzyme is likely an acetylcholinesterase (AChE) since it hydrolyzes acetylthiocholine iodide at a higher rate than other substrates, such as butyrylthiocholine and propionylthiocholine, at pH 7.0 and 25 °C, and is inhibited by eserine but not by iso-OMPA. The enzyme exhibited high sensitivity to some of the various pollutants tested. The kinetic properties of this ChE were compared with those of other invertebrate ChEs. |
| Databáze: | OpenAIRE |
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