Impaired function of CD4CD25 T regulatory lymphocytes characterizes the self-limited hepatitis A virus infection
| Autor: | Costanza Sbreglia, Simona Altamura, Giuseppe Morelli, Alessandro Perrella, Stella Grattacaso, Luigi Racioppi, Luigi Atripaldi, Tommaso Patarino, Laura Vitiello, Oreste Perrella, Pasquale Bellopede |
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| Přispěvatelé: | Perrella, A., Vitiello, Laura, Atripaldi, L., Sbreglia, C., Grattacaso, S., Bellopede, P., Patarino, T., Morelli, G., Altamura, S., Racioppi, Luigi, Perrella, O. |
| Rok vydání: | 2008 |
| Předmět: |
Adult
Male CD3 Complex CD3 Lymphocyte Activation T-Lymphocytes Regulatory DENDRITIC CELLS DISEASE Flow cytometry INFLAMMATION medicine Humans IMMUNE-RESPONSE IL-2 receptor Seroconversion Cells Cultured SUPPRESSION Hepatitis Hepatology biology medicine.diagnostic_test business.industry Interleukin-2 Receptor alpha Subunit Gastroenterology Case-control study T helper cell Hepatitis A medicine.disease Hepatitis a virus medicine.anatomical_structure Case-Control Studies CD4 Antigens AUTOIMMUNE HEPATITIS Immunology biology.protein Female business Hepatitis A Virus Human |
| Zdroj: | Journal of Gastroenterology and Hepatology. 23:e105-e110 |
| ISSN: | 1440-1746 0815-9319 |
| Popis: | Background and Aim: Hepatitis A virus (HAV) causes a transient illness leaving permanent protection against reinfection. Few data are available on the regulatory mechanisms involved in the CD4+ T helper activation. We aimed to investigate the frequency and function of CD3+/CD4+/CD25+ T cells with regulatory function (Tregs) during acute HAV infection. Methods: We enrolled 35 consecutive patients and 15 healthy donors, enumerated Tregs by flow cytometry assay and evaluated, after immunomagnetical sorting with magnetic beads, their ability to inhibit the proliferation of CD4+/CD25– T lymphocytes at different ratios (1:1, 1:10, 1:20). Results: All patients had the usual course of infection. Our immunological analysis showed Tregs frequency in these patients (6.5% [range, 5–8.8%]; 36 [range, 10–87] cells) did not have any statistical difference compared with healthy donors (6% [range, 5–8%]; 48 (range, 23–71) cells), while their ability to suppress CD4+/CD25– was drastically reduced at different ratios (Mann–Whitney U-test; ratio 1:1, 93% vs 72%, z = −3.34, P |
| Databáze: | OpenAIRE |
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