Chronoamperometry Measurement for Rapid Cucumber Mosaic Virus Detection in Plants
| Autor: | A.A. Shahrul, A.R. Rafidah, S. Faridah, M. Mazidah, I. Zamri |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
Chemistry(all) Analytical chemistry 02 engineering and technology medicine.disease_cause 01 natural sciences Cross-reactivity Rapid detection Cucumber mosaic virus medicine Sandwich immunoassay Cucumber Mosaic Virus immunosensor Detection limit Chromatography Chemistry General Medicine Chronoamperometry 021001 nanoscience & nanotechnology chronoamperometry Standard curve Specific antibody plant disease detection Chemical Engineering(all) 0210 nano-technology 010606 plant biology & botany |
| Zdroj: | Procedia Chemistry. 20:25-28 |
| ISSN: | 1876-6196 |
| DOI: | 10.1016/j.proche.2016.07.003 |
| Popis: | Cucumber mosaic virus (CMV) causes major losses to agricultural and horticultural crops around the world. Hence, a rapid assay for the detection of CMV which can be employed in both laboratory and field is essential. A portable electrochemical immunosensor system for the detection of CMV, based on immobilized CMV specific antibodies conjugated with gold nanoparticle was developed for this purpose. The conjugated antibodies were added with polymer and deposited onto carbon screen printed working electrodes. Optimization of the modified surface immunosensor was performed using sandwich immunoassay format (ELISA). The initial ELISA result for the standard curve development showed a limit of detection down to 0.1mg/mL. Subsequently, the immunosensor was tested for cross reactivity with other plant pathogens. The performance of the electrochemical immunosensor revealed that it has a high selectivity in sample matrix with other organism. This immunosensor provides a promising technology for simple and sensitive detection system that is essential in rapid detection of plant pathogens. |
| Databáze: | OpenAIRE |
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