A paradoxical relationship between Resveratrol and copper (II) with respect to degradation of DNA and RNA
| Autor: | Indraneel Mittra, Naveen K Nair, Aishwarya Iyer, Siddharth Subramaniam, Iqbal Vohra |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
plasmid DNA cleavage Resveratrol Biology Cleavage (embryo) pro-oxidant activity General Biochemistry Genetics and Molecular Biology Microbiology 03 medical and health sciences chemistry.chemical_compound RNA degradation Cleave Observation Article Structure: RNA General Pharmacology Toxicology and Pharmaceutics chemistry.chemical_classification Reactive oxygen species General Immunology and Microbiology RNA Articles plasmid DNA degradation General Medicine eukaryotic DNA degradation genomic DNA 030104 developmental biology chemistry Biochemistry Polyphenol copper DNA Cell Signaling & Trafficking Structures Membranes & Sorting |
| Zdroj: | F1000Research |
| ISSN: | 2046-1402 |
| DOI: | 10.12688/f1000research.7202.2 |
| Popis: | Resveratrol (R), a plant polyphenol, is known to reduce Cu (II) to Cu (I) generating reactive oxygen species that can cleave plasmid DNA. Here we report a surprising observation of a paradoxical relationship between R and Cu whereby plasmid DNA cleaving / degrading activity of R-Cu increased progressively as the ratio of R to Cu was increased i.e., the concentration of Cu was successively reduced with respect to a fixed concentration R. Whereas cleavage of plasmid DNA occurred at low molar ratios of R to Cu, at higher ratios, complete degradation of DNA was achieved. By further increasing the ratio, whereby the concentration of Cu was reduced to very low levels, the DNA degrading activity of R-Cu was lost. This paradoxical relationship is also seen with respect to eukaryotic genomic DNA and RNA. Since R-Cu may have anti-cancer and anti-viral activities, our findings may not only help to improve the therapeutic efficacy of R-Cu but also reduce its toxic side effects with the use of low concentration of Cu. |
| Databáze: | OpenAIRE |
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