Potential use of iontophoresis for transdermal delivery of NF-kappaB decoy oligonucleotides
| Autor: | Hidetoshi Arima, Mohamed H. El-Shabouri, Keiichi Motoyama, Irhan Ibrahim Abu Hashim, Abd ElGawad Helmy Abd-ElGawad, Thanaa Mohamed Borg |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Time Factors Oligonucleotides Pharmaceutical Science Dermatitis Pharmacology Administration Cutaneous environment and public health Permeability chemistry.chemical_compound Mice Dermis medicine Transdermal delivery Animals RNA Messenger Fluorescein isothiocyanate skin and connective tissue diseases Transdermal Skin Mice Inbred BALB C Iontophoresis Chemistry Tumor Necrosis Factor-alpha NF-kappa B NF-κB hemic and immune systems NF-κB decoy Skin inflammation Genetic Therapy respiratory system In vitro biological factors Mice Inbred C57BL Disease Models Animal medicine.anatomical_structure Immunology Diffusion Chambers Culture Tetradecanoylphorbol Acetate Tumor necrosis factor alpha Female Decoy |
| Zdroj: | International journal of pharmaceutics. 393(1-2) |
| ISSN: | 1873-3476 0378-5173 |
| Popis: | Topical application of nuclear factor-kappaB (NF-kappaB) decoy appears to provide a novel therapeutic potency in the treatment of inflammation and atopic dermatitis. However, it is difficult to deliver NF-kappaB decoy oligonucleotides (ODN) into the skin by conventional methods based on passive diffusion because of its hydrophilicity and high molecular weight. In this study, we evaluated the in vitro transdermal delivery of fluorescein isothiocyanate (FITC)-NF-kappaB decoy ODN using a pulse depolarization (PDP) iontophoresis. In vitro iontophoretic experiments were performed on isolated C57BL/6 mice skin using a horizontal diffusion cell. The apparent flux values of FITC-NF-kappaB decoy ODN were enhanced with increasing the current density and NF-kappaB decoy ODN concentration by iontophoresis. Accumulation of FITC-NF-kappaB decoy ODN was observed at the epidermis and upper dermis by iontophoresis. In mouse model of skin inflammation, iontophoretic delivery of NF-kappaB decoy ODN significantly reduced the increase in ear thickness caused by phorbol ester as well as the protein and mRNA expression levels of tumor necrosis factor-alpha (TNF-alpha) in the mice ears. These results suggest that iontophoresis is a useful and promising enhancement technique for transdermal delivery of NF-kappaB decoy ODN. |
| Databáze: | OpenAIRE |
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