Synergistic interaction of eugenol and antimicrobial drugs in eradication of single and mixed biofilms of Candida albicans and Streptococcus mutans

Autor:	Mohd Sajjad Ahmad Khan, Hussein H. Abulreesh, Gopa Banerjee, Iqbal Ahmad, Huma Jafri, Abdullah Safar Althubiani
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	FICI SMIC lcsh:Biotechnology Biophysics lcsh:QR1-502 Applied Microbiology and Biotechnology lcsh:Microbiology Microbiology 03 medical and health sciences chemistry.chemical_compound C. albicans lcsh:TP248.13-248.65 medicine PMIC Viability assay Biofilm eradication Candida albicans 030304 developmental biology 0303 health sciences biology 030306 microbiology Biofilm Correction biology.organism_classification Antimicrobial Streptococcus mutans Corpus albicans Eugenol Synergy chemistry Original Article Fluconazole S. mutans medicine.drug
Zdroj:	AMB Express, Vol 10, Iss 1, Pp 1-9 (2020) AMB Express
ISSN:	2191-0855
DOI:	10.1186/s13568-020-01123-2
Popis:	In vitro eradication of the C. albicans and S. mutans mixed biofilms by eugenol alone and in combination with the antimicrobial drugs. Previously characterized strains of C. albicans (CAJ-01 and CAJ-12) and S. mutans MTCC497 were used to evaluate the eradication of biofilms using XTT reduction assay, viability assay, time dependent killing assay and scanning electron microscopy (SEM). Synergistic interaction was assessed by checkerboard method. Sessile MIC (SMIC) of eugenol was equivalent to the planktonic MIC (PMIC) against C. albicans and S. mutans mixed biofilms. SMIC of fluconazole and azithromycin was increased upto 1000-folds over PMIC. Eradication of single or mixed biofilms was evident from the viability assay and SEM. At 1 × MIC of eugenol, log10CFU count of C. albicans cells were decreased from 6.3 to 4.2 and 3.8 (p C. albicans and S. mutans cells from glass surface at 800 µg/mL concentration of eugenol. Time dependent killing assay showed dose dependent effect of eugenol on pre-formed CAJ-01, CAJ-12 and S. mutans biofilm cells. Eugenol was highly synergistic with fluconazole (FICI = 0.156) against CAJ-12 single biofilms. However, the combination of eugenol and azithromycin showed maximum synergy (FICI = 0.140) against pre-formed C. albicans and S. mutans mixed biofilms. These findings highlighted the promising efficacy of eugenol in the eradication of biofilms of two oral pathogens (C. albicans and S. mutans) in vitro and could also be exploited in synergy with fluconazole and azithromycin in controlling oral infections.
Databáze:	OpenAIRE
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