A simple automated injection technique for the high-pressure liquid chromatographic determination of plasma retinol
| Autor: | David W. Howells, I.R.F. Brown, Brooke Og, V Newey |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Adult
Male 030213 general clinical medicine Aging Clinical Biochemistry 030209 endocrinology & metabolism AutoAnalyzer High-performance liquid chromatography law.invention 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Column chromatography law Humans Vitamin A Chromatography High Pressure Liquid Aged Chromatography Autoanalysis Chemistry Extraction (chemistry) Retinol General Medicine Plasma Injector Dilution Circadian Rhythm Female |
| Zdroj: | Annals of clinical biochemistry. 20 |
| ISSN: | 0004-5632 |
| Popis: | We describe a rapid automated method for determination of retinol in 100 μl plasma. A lipid extract of plasma is analysed by reverse phase high pressure liquid chromatography using methanol-water as eluent. Automation of injection is achieved by adaptation of a Technicon AutoAnalyzer Sampler 2 coupled with a Micromedic dilution system and a pneumatically actuated loop injector. Twenty patients' specimens can be analysed in 4 hours, including extraction time, standards, and quality controls, with one extract injected every 4 minutes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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