Optimization of on-line solid phase extraction and HPLC conditions using response surface methodology for determination of WM-5 in mouse plasma and its application to pharmacokinetic study
| Autor: | Meng Wu, Liang Sun, Zheng Yin, Lei Liu, Yaxin Lu, Qing-Ming Wang, Gui-Fang Han, Kang-Ning Liu, Ya-Bin Wen |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
Accuracy and precision Calibration curve Clinical Biochemistry Analytical chemistry Biochemistry High-performance liquid chromatography Analytical Chemistry Mice Drug Stability Ciprofloxacin Limit of Detection Animals Pyrroles Solid phase extraction Response surface methodology Chromatography High Pressure Liquid Detection limit Chromatography Models Statistical Chemistry Extraction (chemistry) Solid Phase Extraction Linearity Reproducibility of Results Cell Biology General Medicine Isoquinolines Linear Models |
| Zdroj: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. |
| ISSN: | 1873-376X |
| Popis: | Response surface methodology (RSM) was utilized for rapid and systematic optimization of on-line solid-phase extraction (SPE) parameters to maximize the response and separation of WM-5. The optimization was performed with Box–Behnken designs. Four major parameters were investigated for their contributions to the response and separation of WM-5, with a total of 29 experiments being performed for each instrument, respectively. Quantitative determination of WM-5 in mouse plasma was performed to evaluate the statistical significance of the parameters on chromatographic response. A fully automated on-line SPE and high-performance liquid chromatography (HPLC) with diode array detection (DAD) method was developed for the determination of WM-5 in mouse plasma. Calibration curve with good linearity ( r = 0.9989) was obtained in the range of 20–4000 ng/mL in mouse plasma. The limit of detection (LOD) and lower limit of quantification (LLOQ) of the assay were 6 ng/mL and 20 ng/mL, respectively. The overall intra-day and the inter-day variations were less than 1.90%. The recovery of the method was in the range of 93.74–96.33% with RSD less than 3.06%. The optimized method demonstrated good performance in terms of specificity, LLOQ, linearity, recovery, precision and accuracy, and was successfully applied to quantify WM-5 in mouse plasma to support the pharmacokinetic study. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect