Isolation and characterization of fatty acid-binding protein from rat brain
Autor: | Kuni Takahashi, Masanori Senjo, Teruo Ishibashi, Teruo Ono, Yoh Imai |
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Rok vydání: | 1985 |
Předmět: |
Immunodiffusion
Chemical Phenomena Size-exclusion chromatography Biophysics Nerve Tissue Proteins Biology Fatty Acid-Binding Proteins Biochemistry Binding Competitive Fatty acid-binding protein Cytosol Animals Molecular Biology Brain Chemistry Isoelectric focusing Binding protein Rats Inbred Strains Neoplasm Proteins Rats Chemistry Isoelectric point Liver biology.protein Chromatography Gel Antibody Isoelectric Focusing Carrier Proteins Fatty Acid-Binding Protein 7 |
Zdroj: | Archives of biochemistry and biophysics. 236(2) |
ISSN: | 0003-9861 |
Popis: | A fatty acid-binding protein has been identified and isolated from the cytosol fraction of rat brain. The fatty acid-binding protein was purified to homogeneity by gel filtration and preparative isoelectric focusing. The binding protein was different from Z protein from rat liver in its isoelectric point and immunological reactivity, in spite of its similar molecular weight of 12,000. Rabbit antibodies against rat liver Z protein were used to demonstrate that the fatty acid-binding proteins from rat liver and brain are immunologically unrelated, and that no Z protein is present in rat brain cytosol. |
Databáze: | OpenAIRE |
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