Prophylactic treatment with sulphonated immunoglobulin G attenuates development of mechanical allodynia-like response in mice with neuropathic pain
| Autor: | Yasuhiro Itano, Daishiro Miura, Yoshinori Kasahara, Wataru Yamamoto, Tsunefumi Kobayashi |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Pharmacology Real-Time Polymerase Chain Reaction Immunoglobulin G Proinflammatory cytokine Mice mechanical allodynia-like response 03 medical and health sciences 0302 clinical medicine Animals Humans Medicine Infusions Parenteral partial sciatic nerve ligation neuropathic pain Full Paper General Veterinary biology business.industry Monocyte medicine.disease sulphonated immunoglobulin G 030104 developmental biology medicine.anatomical_structure Hyperalgesia Immunology Neuropathic pain Neuralgia biology.protein Cytokines Tumor necrosis factor alpha Sciatic nerve medicine.symptom business 030217 neurology & neurosurgery |
| Zdroj: | The Journal of Veterinary Medical Science |
| ISSN: | 1347-7439 0916-7250 |
| DOI: | 10.1292/jvms.15-0195 |
| Popis: | Human immunoglobulin G (IgG) concentrates are immune-modulating, anti-inflammatory plasma-derived products. Clinical studies in recent years have suggested that IgG attenuates neuropathic pain. In this study, effects of sulphonated IgG on the development and maintenance of a mechanical allodynia-like response were examined in mice with neuropathic pain induced by a partial sciatic nerve ligation (PSL). When sulphonated IgG (400 or 1,000 mg/kg/day, i.p.) was administered for 5 days, from 1 day before surgery to post-operative day (POD) 3, the development of a mechanical allodynia-like response was attenuated. On the other hand, sulphonated IgG had little effect on the maintenance of a mechanical allodynia-like response when administered for 5 days, from POD 11 to POD 15, at which time a mechanical allodynia-like response had already been developed. To explore the mechanism of sulphonated IgG, the mRNA expression of inflammatory cytokines was evaluated in the injured sciatic nerve. Sulphonated IgG (1,000 mg/kg/day, i.p.) that was administered for 3 days, from 1 day before surgery to POD 1, significantly attenuated the up-regulation of tumor necrosis factor-α and monocyte chemotactic protein-1 mRNAs on POD 1. These results suggest that prophylactic treatment with sulphonated IgG attenuates the development of mechanical allodynia-like response by inhibition of inflammatory cytokine expression in mice with PSL. |
| Databáze: | OpenAIRE |
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