Autochthonous Bacillus licheniformis: Probiotic potential and survival ability in low‐fishmeal extruded pellet aquafeed
| Autor: | Kang-Woong Kim, Bong-Joo Lee, Soo-Ki Kim, Woo-Do Lee, Sanaz Khosravi, Kai-Min Niu, Jeongmin Lim, Hyon-Sob Han, Sang-Min Lee, Damini Kothari |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Hot Temperature olive flounder Soybean meal lcsh:QR1-502 Cellulase Aquaculture Microbiology lcsh:Microbiology law.invention survival ability 03 medical and health sciences Probiotic 0404 agricultural biotechnology Fish meal law low‐fishmeal aquafeed Bacillus licheniformis Animals Amylase Food science Spores Bacterial biology Chemistry Probiotics fungi 04 agricultural and veterinary sciences Original Articles biology.organism_classification 040401 food science Animal Feed Olive flounder Spore 030104 developmental biology Biofilms biology.protein Flatfishes Original Article Food Additives |
| Zdroj: | MicrobiologyOpen MicrobiologyOpen, Vol 8, Iss 6, Pp n/a-n/a (2019) |
| ISSN: | 2045-8827 |
| Popis: | In recent years, Bacillus spp. have garnered attention as probiotic supplements in aquafeed owing to the production of heat stable and low pH resistant spores. Herein, we isolated and characterized an autochthonous Bacillus licheniformis KCCM 43270 from the intestine of olive flounder (Paralichthys olivaceus) for supplementation in low‐fishmeal extruded aquafeeds. The KCCM 43270 was screened based on amylase, protease, cellulase, and lipase as well as non‐hemolytic activities. The isolate was able to grow in carboxymethyl cellulose (CMC), xylan, and soybean meal (SBM) when used as a single carbon source in the minimal nutrient M9 medium. The KCCM 43270 spores displayed complete survival in acid (pH 2.5) and bile (0.3%, w/v) for 3 hr, strong biofilm formation, and nearly 50% adhesion with intestinal mucus. The spores of the isolate also showed significant survival ability at 80, 90, 100°C for 60, 30, and 1 min, respectively. In addition, the spores in a blend of SBM complex carrier showed significant heat stability at 120°C for 5 min and under different drying conditions. Furthermore, the spores also survived the extrusion process during low‐fishmeal aquafeed manufacturing, implying the potential application of B. licheniformis KCCM 43270 in aquafeed industry. |
| Databáze: | OpenAIRE |
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