Photocrosslinkable hyaluronan as a scaffold for articular cartilage repair
| Autor: | Meredith T. Morgan, Dana L. Nettles, Lori A. Setton, Mark W. Grinstaff, T. Parker Vail |
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| Rok vydání: | 2004 |
| Předmět: |
Scaffold
Compressive Strength Light Biomedical Engineering Cell Culture Techniques Biocompatible Materials Bone matrix Diffusion Chondrocytes In vivo Materials Testing Articular cartilage repair medicine Animals Hyaluronic Acid Cartilage repair Cells Cultured Cell Size Tissue Engineering Chemistry Viscosity Cartilage Hydrogels In vitro Elasticity medicine.anatomical_structure Cross-Linking Reagents Self-healing hydrogels Methacrylates Cartilage Diseases Porosity Cell Division Biomedical engineering |
| Zdroj: | Annals of biomedical engineering. 32(3) |
| ISSN: | 0090-6964 |
| Popis: | Hyaluronan-based scaffolds are of interest for tissue-engineered cartilage repair due to an important role for hyaluronan in cartilage development and function. In this study, an in situ photocrosslinkable hyaluronan (HA-MA) was developed and evaluated as a scaffold for articular cartilage repair. Chondrocytes were encapsulated in crosslinked HA-MA and evaluated for their ability to synthesize cartilaginous matrix in vitro. The mechanical and physical properties of the crosslinked HA-MA hydrogels were similar to that of other hydrogels, with compressive and dynamic shear moduli of 0.6 and 0.3 kPa, respectively, and diffusion coefficients of 600-8000 microm2/s depending on molecular weight. Chondrocytes remained rounded in the HA-MA hydrogels in vitro, and accumulated significant amounts of cartilaginous matrix. Osteochondral defects filled with HA-MA were infiltrated with cells, appeared to integrate well with native tissue, and also accumulated substantial cartilaginous matrix by 2 weeks after surgery. In summary, photocrosslinkable HA-MA promoted the retention of the chondrocytic phenotype and cartilage matrix synthesis for encapsulated chondrocytes in vitro and accelerated healing in an in vivo osteochondral defect model. |
| Databáze: | OpenAIRE |
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