Melatonin regulates ATP content and fertilising capacity of Onychostoma macrolepis spermatozoa by inhibiting ROS accumulation during semen storage in vitro
| Autor: | Qingfang Sun, Jinmeng Yang, Chenhao Yang, Hong Ji, Daqian Dong, Wuzi Dong, Yao Gao, Guofan Peng |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
endocrine system Antioxidant medicine.medical_treatment Cyprinidae Semen Reproductive technology Biology medicine.disease_cause Antioxidants Andrology Melatonin 03 medical and health sciences Endocrinology Adenosine Triphosphate Genetics medicine Animals Molecular Biology reproductive and urinary physiology Sperm motility 030304 developmental biology 0303 health sciences urogenital system Artificial insemination 0402 animal and dairy science 04 agricultural and veterinary sciences 040201 dairy & animal science Sperm Spermatozoa Reproductive Medicine Fertilization Sperm Motility Animal Science and Zoology Reactive Oxygen Species Oxidative stress Developmental Biology Biotechnology medicine.drug Semen Preservation |
| Zdroj: | Reproduction, fertility, and development. 32(14) |
| ISSN: | 1031-3613 |
| Popis: | Melatonin (MLT) is an efficient antioxidant that protects spermatozoa against damages caused by oxidative stress. In this study, to maintain good function of Onychostoma macrolepis spermatozoa during semen preservation invitro at 4°C, different concentrations of MLT (0.5, 1 and 2μM) were added to the semen. After storage (0, 24, 48 and 72h), 1μM MLT in semen markedly improved sperm quality, as reflected by better plasma membrane integrity, the relative steady level of reactive oxygen species (ROS) and slower rate of decrease in mitochondrial membrane potential. Activated spermatozoa in semen with 1μM MLT had higher kinematic performance (i.e. percentage of motile and progressive spermatozoa and the beat cross frequency; P |
| Databáze: | OpenAIRE |
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