Mitochondrial DNA analysis and numerical chromosome condition in human oocytes and polar bodies
| Autor: | Anna Maria Crivello, Anna Pia Ferraretti, Sara De Fanti, Luca Gianaroli, Donata Luiselli, Martin Lang, M. Cristina Magli, Giovanni Romeo |
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| Přispěvatelé: | Gianaroli, Luca, Luiselli, Donata, Crivello, Anna Maria, Lang, Martin, Ferraretti, Anna Pia, De Fanti, Sara, Cristina Magli, M., Romeo, Giovanni |
| Rok vydání: | 2014 |
| Předmět: |
Meiosi
Adult Oocyte Embryology Mitochondrial DNA Haplogroup N Haplogroup H Aneuploidy Polar Bodies Biology Chromosome DNA Mitochondrial Haplogroup Chromosomes Genetic Genetics medicine Humans Oocyte aneuploidy Molecular Biology business.industry Polar Bodie Obstetrics and Gynecology Cell Biology General Medicine medicine.disease Molecular biology Heteroplasmy Hypervariable region Reproductive Medicine Oocytes Female business Infertility Female Human Developmental Biology |
| Zdroj: | Molecular human reproduction. 21(1) |
| ISSN: | 1460-2407 |
| Popis: | To investigate the mitochondrial DNA (mtDNA) segregation in human oocytes, the level of heteroplasmy in the three products of meioses, polar bodies (PBs) and corresponding oocytes, was assessed by studying the hypervariable region I (HVRI) of the D-loop region. The DNA from 122 PBs and 51 oocytes from 16 patients was amplified by whole genome amplification (WGA). An aliquot of the WGA product was used to assess aneuploidy, and another aliquot to study mtDNA. The HVRI was amplified and sequenced with an efficiency of 75.4 and 63%, respectively, in PBs, and of 100% in oocytes. The comparison with the mtDNA sequences from blood of the individual donors showed full correspondence of polymorphisms with the matching oocytes, whilst in PBs the degree of concordance dropped to 89.6%. Haplogroups were inferred for all 16 patients. Of the 89 diagnosed PBs from the 13 patients belonging to macrohaplogroup R, 23 were euploid and 66 aneuploid. The incidence of total anomalies was significantly lower in haplogroup H (6.5%) when compared with haplogroups J and T (17.6 and 13.4% respectively; P < 0.001). In haplogroup J, hypoaneuploidy occurred more frequently than hyperaneuploidy. In the three patients belonging to haplogroup N*, 81% of PBs were aneuploid with similar rates of chromosome hypoaneuploidy and hyperaneuploidy. The presence of mtDNA base changes confined to PBs could reflect a selection mechanism against severe mtDNA mutations, while permitting a high evolution rate that could result in bioenergetic diversity. The different susceptibility to aneuploidy by some haplogroups strongly supports this hypothesis. |
| Databáze: | OpenAIRE |
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