Identification of mature and immature human thymic dendritic cells that differentially express HLA-DR and interleukin-3 receptor in vivo

Autor: Jean Claude Gluckman, Marie-José Alpha, Christian Schmitt, Hélène Fohrer, A H Dalloul, Sylvie Beaudet, Pierre Palmer, Bruno Canque
Přispěvatelé: laboratoire d'immunologie cellulaire et tissulaire (UMR7627), Centre National de la Recherche Scientifique (CNRS), School of Geosciences [Edinburgh], University of Edinburgh, CHU Pitié-Salpêtrière [APHP], École pratique des hautes études (EPHE)
Rok vydání: 2000
Předmět:
Antigens
Differentiation
T-Lymphocyte
Myeloid
medicine.medical_treatment
[SDV]Life Sciences [q-bio]
MESH: Membrane Glycoproteins
CD34
MESH: Antigens
Neoplasm
Antigens
CD34
[SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity
MESH: Cadherins
MESH: Hematopoietic Stem Cells
0302 clinical medicine
MESH: Receptors
Granulocyte-Macrophage Colony-Stimulating Factor
MESH: Reverse Transcriptase Polymerase Chain Reaction
MESH: Vesicular stomatitis Indiana virus
Immunology and Allergy
Myeloid Cells
MESH: Antigens
CD
Cells
Cultured
0303 health sciences
Lymphokines
Membrane Glycoproteins
MESH: Dendritic Cells
Reverse Transcriptase Polymerase Chain Reaction
MESH: Infant
Newborn
Cell Differentiation
Cadherins
Fetal Blood
MESH: Gene Expression Regulation
Cytokine
medicine.anatomical_structure
[SDV.IMM.IA]Life Sciences [q-bio]/Immunology/Adaptive immunology
Receptors
Granulocyte-Macrophage Colony-Stimulating Factor
MESH: Interferon-alpha
MESH: Cells
Cultured
MESH: Cell Differentiation
MESH: Interleukins
MESH: Immunophenotyping
MESH: Interferon-gamma
CD14
Immunology
Interleukin-3 Receptor alpha Subunit
[SDV.CAN]Life Sciences [q-bio]/Cancer
Thymus Gland
Biology
MESH: Receptors
Interleukin-3
Respirovirus
Vesicular stomatitis Indiana virus
Immunophenotyping
03 medical and health sciences
Interferon-gamma
Th2 Cells
Antigen
MESH: Th2 Cells
Antigens
CD
Antigens
Neoplasm
medicine
HLA-DR
Humans
MESH: Fetal Blood
Cell Lineage
030304 developmental biology
MESH: Interleukin-3 Receptor alpha Subunit
CD40
MESH: Humans
MESH: Lymphokines
Interleukins
Infant
Newborn
Interferon-alpha
Cell Biology
MESH: Antigens
CD34
MESH: Thymus Gland
Dendritic Cells
HLA-DR Antigens
MESH: Cell Lineage
MESH: HLA-DR Antigens
Hematopoietic Stem Cells
Molecular biology
MESH: Myeloid Cells
Receptors
Interleukin-3
MESH: Respirovirus
MESH: Antigens
Differentiation
T-Lymphocyte
Gene Expression Regulation
biology.protein
MESH: Biomarkers
Interleukin-3 receptor
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Biomarkers
030215 immunology
Zdroj: Journal of Leukocyte Biology
Journal of Leukocyte Biology, Society for Leukocyte Biology, 2000, 68 (6), pp.836-44. ⟨10.1189/jlb.68.6.836⟩
ISSN: 0741-5400
Popis: We have previously shown that thymic CD34 1 cells have a very limited myeloid differentia- tion capacity and differentiate in vitro mostly into CD1a 1 -derived but not CD14 1 -derived dendritic cells (DC). Herein we characterized the human neo- natal thymic DC extracted from the organ in rela- tionship with the DC generated from CD34 1 cells in situ. We show that in vivo thymic DC express E cad- herin, CLA, CD4, CD38, CD40, CD44, and granu- locyte-macrophage colony-stimulating factor-R (GM- CSF-R; CD116) but no CD1a. According to their morphology, functions, and surface staining they could be separated into two distinct subpopulations: mature HLA-DR hi , mostly interleukin-3-R (CD123)- negative cells, associated with thymocytes, some apoptotic, and expressed myeloid and activation markers but no lymphoid markers. In contrast, immature HLA-DR 1 CD123 hi CD36 1 cells with monocytoid morphology lacked activation and my- eloid antigens but expressed lymphoid antigens. The latter express pTa mRNA, which is also found in CD34 1 thymocytes and in blood CD123 hi DC further linking this subset to lymphoid DC. How- ever, the DC generated from CD34 1 thymic pro- genitors under standard conditions were pTa-neg- ative. Thymic lymphoid DC showed similar pheno- type and cytokine production profile as blood/ tonsillar lymphoid DC but responded to GM-CSF, and at variance with them produced no or little type I interferon upon infection with viruses and did not induce a strict polarization of naive T cells into TH2 cells. Their function in the thymus re- mains therefore to be elucidated. J. Leukoc. Biol. 68: 836-844; 2000.
Databáze: OpenAIRE
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