S-allylmercaptoglutathione Is a Substrate for Glutathione Reductase (E.C. 1.8.1.7) from Yeast (Saccharomyces cerevisiae)
| Autor: | Alan J. Slusarenko, Tobias Horn, Wolfgang Bettray, Martin C.H. Gruhlke |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Physiology Clinical Biochemistry Glutathione reductase Saccharomyces cerevisiae allicin Biochemistry Article thiosulfinate garlic 03 medical and health sciences chemistry.chemical_compound Molecular Biology glutathione reductase chemistry.chemical_classification Growth medium Allicin biology lcsh:RM1-950 food and beverages Substrate (chemistry) Cell Biology Glutathione biology.organism_classification Yeast lcsh:Therapeutics. Pharmacology 030104 developmental biology Enzyme chemistry ddc:540 |
| Zdroj: | Antioxidants 7(7), 86 (2018). doi:10.3390/antiox7070086 special issue: "Special Issue "Feature Papers in Antioxidants in 2018" / Guest Editor: Prof. Dr. Stanley Omaye, Department of Agriculture, Nutrition and Veterinary Sciences, University of Nevada, North Virginia" Antioxidants, Vol 7, Iss 7, p 86 (2018) Antioxidants Volume 7 Issue 7 |
| DOI: | 10.3390/antiox7070086 |
| Popis: | Allicin (diallylthiosulfinate) is a potent thiol reagent and natural defense substance produced by garlic (Allium sativum) tissues when damaged. Allicin acts as a redox toxin and oxidizes the cellular glutathione (GSH) pool producing S-allylmercaptoglutathione (GSSA). The cellular enzyme glutathione reductase (GR) uses NADPH to reduce glutathione disulfide (GSSG) back to GSH and replenishes the GSH pool. It was not known whether GR could accept GSSA as a substrate. Here, we report that GR from yeast (Saccharomyces cerevisiae) shows Michaelis&ndash Menten kinetics with GSSA as substrate in vitro (Km = 0.50 mM), but that GSSA is not as good a substrate as GSSG (Km = 0.07 mM). Furthermore, cells unable to synthesize GSH because the &gamma glutamylcysteine synthetase (GSH1) gene is deleted, cannot grow without GSH supplementation and we show that the auxotrophic requirement for GSH in &Delta gsh1 mutants can be met by GSSA in the growth medium, suggesting that GSSA can be reduced to GSH in vivo. |
| Databáze: | OpenAIRE |
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