Peroxyoxalate chemiluminescence detection for the highly sensitive determination of fluorescence-labeled chlorpheniramine with Suzuki coupling reaction
| Autor: | Naoya Kishikawa, Naotaka Kuroda, Lawrence A. Adutwum, Kaname Ohyama, Shiro Harada, Kenichiro Nakashima |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Chlorpheniramine
Fluorescence spectrometry Biochemistry High-performance liquid chromatography Peroxyoxalate Sensitivity and Specificity Fluorescence Analytical Chemistry law.invention chemistry.chemical_compound Suzuki reaction law Suzuki coupling reaction Humans Solid phase extraction Chromatography High Pressure Liquid Chemiluminescence Fluorescent Dyes Detection limit Oxalates Peroxyoxalate chemiluminescence Chromatography Chemistry Luminescent Measurements HPLC Monodesmethyl chlorpheniramine Boronic acid |
| Zdroj: | Analytical and bioanalytical chemistry. 398(2):823-829 |
| ISSN: | 1618-2642 |
| Popis: | A sensitive and selective high performance liquid chromatography-peroxyoxalate chemiluminescence (PO-CL) method has been developed for the simultaneous determination of chlorpheniramine (CPA) and monodesmethyl chlorpheniramine (MDCPA) in human serum. The method combines fluorescent labeling with 4-(4,5-diphenyl-1H-imidazole-2-yl)phenyl boronic acid using Suzuki coupling reaction with PO-CL detection. CPA and MDCPA were extracted from human serum by liquid-liquid extraction with n-hexane. Excess labeling reagent, which interfered with trace level determination of analytes, was removed by solid-phase extraction using a C18 cartridge. Separation of derivatives of both analytes was achieved isocratically on a silica column with a mixture of acetonitrile and 60 mM imidazole-HNO(3) buffer (pH 7.2; 85:15, v/v) containing 0.015% triethylamine. The proposed method exhibited a good linearity with a correlation coefficient of 0.999 for CPA and MDCPA within the concentration range of 0.5-100 ng/mL. The limits of detection (S/N = 3) were 0.14 and 0.16 ng/mL for CPA and MDCPA, respectively. Using the proposed method, CPA could be selectively determined in human serum after oral administration. Analytical and bioanalytical chemistry, 398(2), pp.823-829; 2010 |
| Databáze: | OpenAIRE |
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