| Autor: |
Qun Ge, Yànli Cūi, Jùnwén Lǐ, Jǔwǔ Gōng, Quánwěi Lú, Péngtāo Lǐ, Yùzhēn Shí, Hǎihóng Shāng, Àiyīng Liú, Xiǎoyīng Dèng, Jìngtāo Pān, Qúanjiā Chén, Youlu Yuan, Wankui Gong |
| Rok vydání: |
2020 |
| DOI: |
10.21203/rs.2.21774/v3 |
| Popis: |
Background：Fructose-1,6-bisphosphatase (FBP) is a key enzyme in plant sucrose synthesis pathway in Calvin cycle and plays an important role in photosynthesis regulation in green plants. However, no systemic analysis of the FBPs has been reported in Gossypium species.Results：A total of 41 FBP genes from four Gossypium species were identified and analyzed. The FBP genes were assorted into two groups and 7 subgroups. The results revealed that FBP family genes were under a purifying selection pressure which rendered FBP family members a conserved evolution pattern and that there was no tandem and fragmental DNA duplication in FBP family genes. Collinearity analysis revealed that a FBP gene was located in the translocated DNA fragment and the whole FBP gene family was under a disequilibrium evolution pattern which led to a faster evolution progress of the members in G. barbadense and in At subgenome than those in the rest Gossypium species and in Dt subgenome, respectively of this study. Through RNA-seq analyses and qRT-PCR verifications, different FBP genes have diversified biological functions in cotton fiber development (2 genes in 0 DPA and 1DPA ovules and 4 genes in 20-25 DPA fibers), and in plant responses to Verticillium wilt onset (2 genes) and to salt stress (8 genes).Conclusion: The FBP gene family displayed disequilibrium evolution pattern in Gossypium species, which render them diversified functions affecting not only fiber development, but also responses to the Verticillium wilt and the salt stress. All the findings provided the foundation for further study of the function of the FBP genes in cotton fiber development and in environmental adaptability. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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