Antimicrobial properties of Kalanchoe blossfeldiana: a focus on drug resistance with particular reference to quorum sensing-mediated bacterial biofilm formation
| Autor: | Jayanta Roy Choudhury, Pulok K. Mukherjee, Tuhinadri Sen, Sanmoy Karmakar, Rajib Barik, Ratul Sarkar, Sumon Chakraborty, Rammohan Bera, Chaitali Mondal, Sushil K Chaudhary, Kirendra Kumar Yadav, Satadal Das, Alekh Kumar, Paramita Roy, Samir K. Samanta, Joydeep Mukherjee |
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| Rok vydání: | 2014 |
| Předmět: |
Kalanchoe
Interleukin-1beta Homoserine Drug Resistance Pharmaceutical Science Virulence Biology medicine.disease_cause Virulence factor Microbiology chemistry.chemical_compound medicine Humans Cells Cultured Pharmacology Pseudomonas aeruginosa Interleukin-6 Plant Extracts Tumor Necrosis Factor-alpha Kalanchoe blossfeldiana Biofilm Quorum Sensing Antimicrobial biology.organism_classification Anti-Bacterial Agents Plant Leaves Quorum sensing Biochemistry chemistry Biofilms Leukocytes Mononuclear |
| Zdroj: | The Journal of pharmacy and pharmacology. 67(7) |
| ISSN: | 2042-7158 |
| Popis: | ObjectivesThis study attempts to investigate the antimicrobial properties of Kalanchoe blossfeldiana with a particular reference to quorum sensing (QS)-mediated biofilm formation.MethodsThe methanol extract of K. blossfeldiana leaves (MEKB) was evaluated for antimicrobial properties including QS-controlled production of biofilm (including virulence factor, motility and lactone formation) in Pseudomonas aeruginosa. Methanol extract of K. blossfeldiana was also evaluated for anti-cytokine (tumour necrosis factor-alpha, interleukin-6 and interleukin-1 beta) properties in peripheral blood mononuclear cells (PBMC).Key findingsMethanol extract of K. blossfeldiana exhibited antimicrobial effect on clinical isolates, as well as standard reference strains. Pseudomonas aeruginosa exposed to MEKB (subminimum inhibitory concentration (MIC)) displayed reduced biofilm formation, whereas supra-MIC produced destruction of preformed biofilms. Methanol extract of K. blossfeldiana reduced the secretion of virulence factors (protease and pyoverdin) along with generation of acyl homoserine lactone (AHL). Confocal laser scanning microscopy images indicate reduction of biofilm thickness. The extract also reduced cytokine formation in lipopolysaccharide-stimulated PBMC.ConclusionsK alanchoe blossfeldiana was found to interfere with AHL production, which in turn may be responsible for downregulating QS-mediated production of biofilm and virulence. This first report on the antibiofilm and anticytokine properties of this plant may open up new vistas for future exploration of this plant for combating biofilm-related resistant infections. |
| Databáze: | OpenAIRE |
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