Regulation of URG4/URGCP and PPARα gene expressions after retinoic acid treatment in neuroblastoma cells

Autor: Cigir Biray Avci, Cumhur Gündüz, Gulsah Gundogdu, Hasan Onur Caglar, N. Lale Satiroglu-Tufan, Vural Kucukatay, Yavuz Dodurga
Rok vydání: 2013
Předmět:
Time Factors
SH-SY5Y
gene amplification
polymerase chain reaction
Cellular differentiation
Retinoic acid
protein binding
PPARα
Proto-Oncogene Mas
Neuroblastoma
chemistry.chemical_compound
URG4/URGCP
oncogene
Gene expression
Neuroblastoma cells
retinoic acid
Regulation of gene expression
neurite
Reverse Transcriptase Polymerase Chain Reaction
article
gene expression regulation
General Medicine
Neoplasm Proteins
Gene Expression Regulation
Neoplastic
priority journal
Differentiation
neuroblastoma cell
GAPDH Gene
URG4 URGCP gene
glyceraldehyde 3 phosphate
PPAR?
gene overexpression
gene rearrangement
Antineoplastic Agents
Tretinoin
Biology
reverse transcription polymerase chain reaction
GAPDH gene
Cell Line
Tumor
Humans
metastasis
PPAR alpha
controlled study
human
Oncogene
human cell
Gene rearrangement
tumor recurrence
Molecular biology
protein phosphorylation
cell differentiation
chemistry
PPAR alpha gene
Zdroj: Tumor Biology. 34:3853-3857
ISSN: 1423-0380
1010-4283
DOI: 10.1007/s13277-013-0970-1
Popis: Neuroblastoma (NB), originating from neural crest cells, is the most common extracranial tumor of childhood. Retinoic acid (RA) which is the biological active form of vitamin A regulates differentiation of NB cells, and RA derivatives have been used for NB treatment. PPARα (peroxisome proliferator-activated receptor) plays an important role in the oxidation of fatty acids, carcinogenesis, and differentiation. URG4/URGCP gene is a proto-oncogene and that overexpression of URG4/URGCP is associated with metastasis and tumor recurrence in osteosarcoma. It has been known that URG4/URGCP gene is an overexpressed gene in hepatocellular carcinoma and gastric cancers. This study aims to detect gene expression patterns of PPARα and URG4/URGCP genes in SH-SY5Y NB cell line after RA treatment. Expressions levels of PPARα and URG4/URGCP genes were analyzed after RA treatment for reducing differentiation in SH-SY5Y NB cell line. To induce differentiation, the cells were treated with 10 μM RA in the dark for 3-10 days. Gene expression of URG4/URGCP and PPARα genes were presented as the yield of polymerase chain reaction (PCR) products from target genes compared with the yield of PCR products from the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene. SH-SY5Y cells possess small processes in an undifferentiated state, and after treatment with RA, the cells developed long neurites, resembling a neuronal phenotype. PPARα gene expression increased in RA-treated groups; URG4/URGCP gene expression decreased in SH-SY5Y cells after RA treatment compared with that in the control cells. NB cell differentiation might associate with PPARα and URG4/URGCP gene expression profile after RA treatment. © 2013 International Society of Oncology and BioMarkers (ISOBM).
Databáze: OpenAIRE
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