Optimization of β-Glucosidase, β-Xylosidase and Xylanase Production by Colletotrichum graminicola under Solid-State Fermentation and Application in Raw Sugarcane Trash Saccharification
| Autor: | Monica Stropa Ferreira Nozawa, Ana Lucia Ribeiro Latorre Zimbardi, João Atílio Jorge, Luana Parras Meleiro, Flavio Henrique Moreira Souza, Rosa dos Prazeres Melo Furriel, Cesar Sehn, Luis Henrique Souza Guimarães, D. C. Masui |
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| Rok vydání: | 2013 |
| Předmět: |
ENZIMAS HIDROLÍTICAS
Cellulase Colletotrichum graminicola Article Catalysis lcsh:Chemistry Inorganic Chemistry Graminicola Enzymatic hydrolysis Ethanol fuel Food science Physical and Theoretical Chemistry lcsh:QH301-705.5 Molecular Biology Spectroscopy Trichoderma reesei β-glucosidase β-xylosidase xylanase sugarcane trash hydrolysis biology Chemistry Beta-glucosidase Organic Chemistry General Medicine biology.organism_classification Computer Science Applications lcsh:Biology (General) lcsh:QD1-999 Solid-state fermentation Agronomy biology.protein Xylanase |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP International Journal of Molecular Sciences, Vol 14, Iss 2, Pp 2875-2902 (2013) International Journal of Molecular Sciences International Journal of Molecular Sciences; Volume 14; Issue 2; Pages: 2875-2902 |
| ISSN: | 1422-0067 |
| Popis: | Efficient, low-cost enzymatic hydrolysis of lignocellulosic residues is essential for cost-effective production of bioethanol. The production of β-glucosidase, β-xylosidase and xylanase by Colletotrichum graminicola was optimized using Response Surface Methodology (RSM). Maximal production occurred in wheat bran. Sugarcane trash, peanut hulls and corncob enhanced β-glucosidase, β-xylosidase and xylanase production, respectively. Maximal levels after optimization reached 159.3 ± 12.7 U g−1, 128.1 ± 6.4 U g−1 and 378.1 ± 23.3 U g−1, respectively, but the enzymes were produced simultaneously at good levels under culture conditions optimized for each one of them. Optima of pH and temperature were 5.0 and 65 °C for the three enzymes, which maintained full activity for 72 h at 50 °C and for 120 min at 60 °C (β-glucosidase) or 65 °C (β-xylosidase and xylanase). Mixed with Trichoderma reesei cellulases, C. graminicola crude extract hydrolyzed raw sugarcane trash with glucose yield of 33.1% after 48 h, demonstrating good potential to compose efficient cocktails for lignocellulosic materials hydrolysis. |
| Databáze: | OpenAIRE |
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