A survey of adenine and 4-aminopyrazolo[3,4-d]pyrimidine (4-APP) as inhibitors of ribosome-inactivating proteins (RIPs)
| Autor: | Lucio Montanaro, Maurizio Brigotti, Domenica Carnicelli, Simona Rizzi, Simonetta Sperti |
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| Rok vydání: | 2001 |
| Předmět: |
endocrine system
Saporin Pyrimidine Ribosome Inactivating Proteins General Biochemistry Genetics and Molecular Biology Shiga Toxin chemistry.chemical_compound medicine Animals General Pharmacology Toxicology and Pharmaceutics Gelonin N-Glycosyl Hydrolases Plant Proteins Protein Synthesis Inhibitors biology Momordin Plant Extracts Ribosome-inactivating protein Adenine Shiga toxin General Medicine Molecular biology Ricin chemistry Biochemistry Mechanism of action biology.protein medicine.symptom Artemia Ribosomes |
| Zdroj: | Life sciences. 68(3) |
| ISSN: | 0024-3205 |
| Popis: | The inhibitory power of adenine and 4-aminopyrazolo[3,4-d]pyrimidine (4-APP) on the RNA-N-glycosidase activity catalyzed by bacterial (Shiga toxin 1) and plant (ricin, gelonin, momordin, bryodin-R, PAP-S, luffin, trichosantin, saporin 6 and barley) RIPs has been compared. The behavior of the two inhibitors is largely variable. While Shiga toxin 1 is preferentially inhibited by 4-APP, plant RIPs are either preferentially inhibited by adenine, or equally inhibited by the two compounds or, finally, only slightly more by 4-APP. Sequence variabilities involved in these different behaviors are discussed. The experimental data clearly indicate that, in spite of the same mechanism of action, RIPs differ widely in the ability to fit small ring molecules in the active cleft. While the strong inhibitory power of 4-APP on Shiga toxin 1 opens perspectives of therapeutic interventions, the ineffectiveness of the compound on ricin precludes its use as a suitable antidote in poisoning. |
| Databáze: | OpenAIRE |
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