Changing the shape of wool yarns via laccase-mediated grafting of tyrosine
Autor: | Carla Silva, Su Jing, Madalena Martins, Yu Li, Artur Cavaco-Paulo, Jennifer Noro |
---|---|
Přispěvatelé: | Universidade do Minho |
Rok vydání: | 2021 |
Předmět: |
Bioengineering
02 engineering and technology 010402 general chemistry 01 natural sciences Applied Microbiology and Biotechnology Polyvinyl alcohol chemistry.chemical_compound Shape modeling Animals Tyrosine grafting Tyrosine chemistry.chemical_classification Laccase Science & Technology Wool General Medicine 021001 nanoscience & nanotechnology Grafting 0104 chemical sciences chemistry Chemical engineering Benzyl alcohol Thiol 0210 nano-technology Wool yarns Biotechnology Cysteine |
Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
ISSN: | 0168-1656 |
Popis: | The shape of wool yarns was changed by laccase-assisted grafting of tyrosine. Prior to tyrosine grafting a cysteine pre-treatment was optimized aiming to increase the amount of thiol reaction groups available. The best operational conditions for laccase-assisted tyrosine grafting were: i) pre-treatment with cysteine (2.2 mM) in a solution of 20 % ethanol, 15 % propylene glycol and 0.5 % benzyl alcohol, pH=10, 40 °C; ii) tyrosine grafting with 3.0 mM tyrosine, 18 U/mL laccase, pH=5, 40 °C. The shape modification was evaluated by number of curly twists determination on the grafted yarn samples. The thermal and mechanical properties of the grafted wool yarns was evaluated by TGA, DSC and breaking strength determination. The amount of free thiols and weight gain were assessed aiming to infer the role of the cysteine pre-treatment on the final tyrosine grafting and shape modification. The laccase-assisted grafting of tyrosine onto wool yarns have influenced the thermal and mechanical properties of the yarns however without compromising their structural integrity for the final application purposes. The developed methodology to impart new shape to wool yarns is presented herein as an environmentally friendly alternative to chemical methods. The new findings revealed great potentialities for application in similar fibers like hair. This study was supported by Chinese Government Scholarship under China Scholar Council [NO. 201906790043] and “the National Nature Science Foundation of China [Grant NO. 52003108]. This study was also supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and BioTecNorte operation [Norte-01-0145-FEDER-000004] funded by the European Regional Development Fund under the scope of Norte2020 – Programa Operacional Regional do Norte. Jennifer Noro thanks to FCT for the funding [grand number SFRH/BD/121673]. info:eu-repo/semantics/publishedVersion |
Databáze: | OpenAIRE |
Externí odkaz: |