Resolution of allelic and non-allelic variants of histone H1 by cation-exchange-hydrophilic-interaction chromatography
| Autor: | Andrew J. Alpert, Theo P.A. Kruck, Lyne Levesque, Craig Andrew Mizzen, Donald R. McLachlan |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
chemistry.chemical_classification
Quality Control Chromatography Acetonitriles Erythrocytes Polymorphism Genetic Resolution (mass spectrometry) Sequence Homology Amino Acid Hydrophilic interaction chromatography Molecular Sequence Data General Chemistry Chromatography Ion Exchange High-performance liquid chromatography Amino acid Histones Histone H1 chemistry Polymorphism (computer science) Animals Amino Acid Sequence Polyacrylamide gel electrophoresis Peptide sequence Chickens Alleles Chromatography High Pressure Liquid |
| Zdroj: | Journal of chromatography. B, Biomedical sciences and applications. 744(1) |
| ISSN: | 1387-2273 |
| Popis: | A mixed-mode high-performance liquid chromatography (HPLC) method that resolves the six known non-allelic variants of chicken erythrocyte histone H1 is described. Common, but previously unknown, allelic variants of H1 that comigrate in polyacrylamide gel electrophoresis are also resolved. The resolution of H1 variants achieved by this method should be useful in determining the functional significance of H1 sequence heterogeneity and in analyses of post-translational modification of H1. Furthermore, the principles behind the separation should be applicable to analyses of polymorphism in other proteins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect