In Situ Expression of Regulatory Cytokines by Heart Inflammatory Cells in Chagas' Disease Patients with Heart Failure
| Autor: | Virmondes Rodrigues, Denise Bertulucci Rocha Rodrigues, Audrey Romano, Vicente de Paula Antunes Teixeira, Sebastião Tostes Junior, Sanívia Aparecida de Lima Pereira, Marlene Antônia dos Reis |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
lcsh:Immunologic diseases. Allergy
Chagas disease Pathology medicine.medical_specialty Article Subject Immunology Endomyocardial fibrosis Nitric Oxide Synthase Type II Biology Interferon-gamma Immune system Th2 Cells parasitic diseases medicine Immunology and Allergy Humans Interferon gamma Chagas Disease Heart Failure Tumor Necrosis Factor-alpha FOXP3 General Medicine Th1 Cells medicine.disease Endomyocardial Fibrosis Heart failure biology.protein Clinical Study Cytokines Tumor necrosis factor alpha Antibody lcsh:RC581-607 medicine.drug |
| Zdroj: | Clinical and Developmental Immunology Clinical and Developmental Immunology, Vol 2012 (2012) |
| ISSN: | 1740-2530 1740-2522 |
| Popis: | Chagas' disease is caused by the protozoan parasiteTrypanosoma cruzi. The immune system plays an important role in the reduction of parasite load, but may also contribute to the development of lesions observed during the chronic phase of the disease. We analyzed cytokines produced by inflammatory heart cells in 21 autopsy samples obtained from patients with Chagas' disease divided according to the presence or absence of heart failure (HF). Left ventricular sections were analyzed by immunohistochemistry using antibodies against human IL-4, IFN-γ, TGF-β, TNF-α, and NOS2.In situmRNA expression was quantified by a Low Density Array. The number of IFN-γ-positive cells was significantly higher than IL-4 positive cells. TNF-α, TGF-βand NOS2 were detected in 65%, 62% and 94% of samples respectively. There was an association between TNF-α-producing cells and the presence of HF. Subjects with HF presented higher levels of STAT4 mRNA, whereas FoxP3 and STAT6 levels were similar in the two groups. A Th1 cytokine pattern predominated in the cardiac inflammatory cell infiltrate of Chagas’ disease patients associated with HF. High degree of fibrosis was associated with low NOS2 expression. These results support the idea that Th1 immune responses are involved in heart lesions of Chagas' disease patients. |
| Databáze: | OpenAIRE |
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