| Autor: |
Sanmarié Schlebusch, Rikki M. A. Graham, Amy V. Jennison, Melissa M. Lassig-Smith, Patrick N. A. Harris, Jeffrey Lipman, Páraic Ó Cuív, David L. Paterson |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
BMC microbiology. 22(1) |
| ISSN: |
1471-2180 |
| Popis: |
Background The purpose of this study was to investigate the use of routinely available rectal swabs as a surrogate sample type for testing the gut microbiome and monitoring antibiotic effects on key gut microorganisms, of patients hospitalised in an intensive care unit. A metagenomic whole genome sequencing approach was undertaken to determine the diversity of organisms as well as resistance genes and to compare findings between the two sampling techniques. Results No significant difference was observed in overall diversity between the faeces and rectal swabs and sampling technique was not demonstrated to predict microbial community variation. More human DNA was present in the swabs and some differences were observed only for a select few anaerobes and bacteria also associated with skin and/or the female genitourinary system, possibly reflecting sampling site or technique. Antibiotics and collections at different times of admission were both considered significant influences on microbial community composition alteration. Detection of antibiotic resistance genes between rectal swabs and faeces were overall not significantly different, although some variations were detected with a potential association with the number of human sequence reads in a sample. Conclusion Testing the gut microbiome using standard rectal swab collection techniques currently used for multi-resistant organism screening has been demonstrated to have utility in gut microbiome monitoring in intensive care. The use of information from this article, in terms of methodology as well as near equivalence demonstrated between rectal swabs and faeces will be able to support and potentially facilitate the introduction into clinical practice. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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