Comparative proteomic analysis of nuclear and cytoplasmic compartments in human cardiac progenitor cells
Autor: | Albericio, Guillermo, Aguilar, Susana, Torán, Jose Luis, Yañez, Rosa, López, Juan Antonio, Vázquez, Jesús, Mora, Carmen, Bernad, Antonio |
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Přispěvatelé: | Unión Europea. Comisión Europea, Ministerio de Ciencia e Innovación (España), Comunidad de Madrid (España), Instituto de Salud Carlos III |
Jazyk: | angličtina |
Rok vydání: | 2022 |
Předmět: |
Proteomics
Cytoplasm Cell biology Proteome Science Cardiology Article Cell Movement Interleukin-1alpha Humans Myocytes Cardiac Cells Cultured Cell Proliferation Cell Nucleus Multidisciplinary HMGA2 Protein Receptor-Like Protein Tyrosine Phosphatases Class 2 RNA-Binding Proteins Mesenchymal Stem Cells Fibroblasts Oxidative Stress Gene Expression Regulation Medicine Signal Transduction |
Zdroj: | Scientific Reports, Vol 12, Iss 1, Pp 1-16 (2022) Scientific Reports |
ISSN: | 2045-2322 |
Popis: | Clinical trials evaluating cardiac progenitor cells (CPC) demonstrated feasibility and safety, but no clear functional benefits. Therefore a deeper understanding of CPC biology is warranted to inform strategies capable to enhance their therapeutic potential. Here we have defined, using a label-free proteomic approach, the differential cytoplasmic and nuclear compartments of human CPC (hCPC). Global analysis of cytoplasmic repertoire in hCPC suggested an important hypoxia response capacity and active collagen metabolism. In addition, comparative analysis of the nuclear protein compartment identified a significant regulation of a small number of proteins in hCPC versus human mesenchymal stem cells (hMSC). Two proteins significantly upregulated in the hCPC nuclear compartment, IL1A and IMP3, showed also a parallel increase in mRNA expression in hCPC versus hMSC, and were studied further. IL1A, subjected to an important post-transcriptional regulation, was demonstrated to act as a dual-function cytokine with a plausible role in apoptosis regulation. The knockdown of the mRNA binding protein (IMP3) did not negatively impact hCPC viability, but reduced their proliferation and migration capacity. Analysis of a panel of putative candidate genes identified HMGA2 and PTPRF as IMP3 targets in hCPC. Therefore, they are potentially involved in hCPC proliferation/migration regulation. THis study was initiated by European Commission funding (HEALTH-2009_242038) and by grants to AB from the Spanish Ministry of Science and Innovation RTI2018-097604-B-I00 (AEI/FEDER, UE) and SAF2015- 70882-R. Te Research Program of the Comunidad Autónoma de Madrid (S2017/BMD-3692) and the Instituto de Salud Carlos III (RETICS-RTI2018-097604-B-I00) to AB also funded parts of the work. We also wish to thank to K McCreath for editorial work. Sí |
Databáze: | OpenAIRE |
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