Simple fabrication method for a porous poly(vinyl alcohol) matrix by multisolvent mixtures for an air-exposed model of the lung epithelial system
| Autor: | Cho Hay Mun, Da Yoon Noh, Sang Hoon Lee, Edward Kang, Su Kyoung Chae |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Vinyl alcohol
Recrystallization (geology) Materials science Surface Properties Models Biological chemistry.chemical_compound Polymer chemistry Electrochemistry General Materials Science Particle Size Porosity Dissolution Lung Spectroscopy Aqueous solution integumentary system Air Isopropyl alcohol Epithelial Cells Surfaces and Interfaces Condensed Matter Physics respiratory tract diseases Solvent chemistry Chemical engineering Polyvinyl Alcohol Solvents Glutaraldehyde |
| Zdroj: | Langmuir : the ACS journal of surfaces and colloids. 30(41) |
| ISSN: | 1520-5827 |
| Popis: | We introduce a simple and easy method for fabricating a thin and porous matrix that can be used as an extracellular matrix (ECM). A porous poly(vinyl alcohol) (PVA) matrix was created through recrystallization by multiple solvents under distilled water (DW), isopropyl alcohol (IPA), and a combination of DW and IPA. The crysatllization was driven by precipitating and dissolving a solute in a solution of a solvent and a nonsolvent, which induced the formation of microspheres in the IPA. The crystal structure depended on the ratio of the solvent/nonsolvent and the concentration of the PVA aqueous solution; these properties were used to tune the thickness, size, and porosity of the matrices. The resulting PVA matrix was chemically stabilized through a reaction with glutaraldehyde in the IPA solution. We demonstrated that a very thin and porous PVA matrix provided an effective functional model of the lung epithelial system. Lung epithelial cells (A549) displayed a high affinity for this matrix, which was permeable to the culture medium. These properties facilitated culturing under the air environment. |
| Databáze: | OpenAIRE |
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