Oleuropein ameliorates arsenic induced oxidative stress in mice
| Autor: | Oğuz Merhan, Ayla Özcan, Hasan Özen, Musa Karaman, Abdulsamed Kükürt, Mahmut Karapehlivan, Metin Ogun |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Sodium arsenite Nitric Oxide Synthase Type III Arsenites Protein Carbonyl Content Iridoid Glucosides Nitric Oxide Synthase Type II 010501 environmental sciences Nitric Oxide medicine.disease_cause Endothelial NOS 01 natural sciences Biochemistry Inorganic Chemistry Mice 03 medical and health sciences chemistry.chemical_compound Oleuropein Enos Malondialdehyde Internal medicine medicine Animals Iridoids 0105 earth and related environmental sciences biology Arsenic toxicity Free Radical Scavengers biology.organism_classification Sodium Compounds Oxidative Stress 030104 developmental biology Endocrinology Liver chemistry Molecular Medicine Oxidative stress |
| Popis: | The objective of this study is to investigate the potential preventive effect of oleuropein in an experimental arsenic toxicity in mice. For this purpose, mice were exposed to 5mg/kg/day sodium arsenite (NaAsO2) in drinking water and treated with 30mg/kg/day oleuropein for 15 days. At the end of the experiment, animals were sacrificed and selected organs were processed for biochemical and histopahtological investigations. Blood, liver, kidney and brain malondialdehyde (MDA) and nitric oxide (NO) levels were determined by colorimetric methods. Protein carbonyl content is measured by a commercial kit. Liver morphology and immunoreactivity for inducible NOS (iNOS) and endothelial NOS (eNOS) was evaluated microscopically. Level of NO was determined to decrease in blood and tissues whereas MDA increased in arsenic given mice. Tissue protein carbonyl content also increased in this group. Immunoreactivity for iNOS and eNOS was noted to increase with arsenic treatment. Oleuropein treatment had significant effects in normalizing the MDA and NO levels as well as protein carbonyl content. Immunohistochemical staining also showed reduction of the expression of iNOS and eNOS in liver. The results indicate that oleuropein ameliorates oxidative tissue damage by scavenging free radicals. |
| Databáze: | OpenAIRE |
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