Marek's disease virus protein kinase gene identified within the short unique region of the viral genome is not essential for viral replication in cell culture and vaccine-induced immunity in chickens
| Autor: | Toyohiko Urakawa, Yumiko Hirayama, Kanji Hirai, Michitaka Yamamoto, Noriko Miki, Masashi Sakaguchi, Geng-Sheng Zhu |
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| Rok vydání: | 1993 |
| Předmět: |
viruses
Genetic Vectors Molecular Sequence Data Restriction Mapping DNA Recombinant Chick Embryo Genome Viral Biology medicine.disease_cause Recombinant virus Virus Replication Virus law.invention Mice Dogs law Virology HSPA2 medicine Marek Disease Animals Amino Acid Sequence Cloning Molecular Phosphorylation Gene Herpesvirus 2 Gallid Cells Cultured Marek's disease Mice Inbred BALB C Base Sequence Viral Vaccines biology.organism_classification Molecular biology Herpes simplex virus Viral replication Lac Operon DNA Viral Recombinant DNA Baculoviridae Chickens Protein Kinases |
| Zdroj: | Virology. 195(1) |
| ISSN: | 0042-6822 |
| Popis: | The open reading frame (ORF) of 1206 bp within the short unique region (Us) of Marek's disease virus type 1 (MDV1) shows significant homology with the herpes simplex virus type 1 US3 gene encoding protein kinase (PK). The lacZ gene of Escherichia coli was inserted within the ORF, designated MDV1-US3, of MDV1 K544 strain DNA by homologous recombination. The plaque-purified recombinant MDV1 stably expressed the β-galactosidase encoded by the inserted lacZ gene in infected cells and replicated well as the parental K544 strain. Antibodies against both MDV1 antigen and β-galactosidase were detected in the sera of chickens immunized with recombinant MDV1. Chickens vaccinated with the recombinant MDV1 were protected from challenge with virulent MDV1. The MDV1 US3 gene expressed by a baculovirus vector encoded a 44-kDa protein. Mouse antisera against the 44-kDa protein reacted with two proteins of 44 and 45 kDa in extracts of cells infected with MDV1 but not with MDV types 2 or 3. The PK activity was detected in immune complexes of the anti-44-kDa sera with extracts of cells infected with MDV1 but not with the recombinant MDV1. Thus, PK encoded from the MDV1-US3 is not essential for virus replication in cell culture and vaccine-induced immunity. |
| Databáze: | OpenAIRE |
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