Indirect ELISA (iELISA) standardization for the diagnosis of bovine enzootic leukosis
| Autor: | Rômulo Cerqueira Leite, Jenner Karlisson Pimenta dos Reis, Telissa da Cunha Kassar, Emília Delarmelina, Raphael M. Victor, Cláudia Fideles Resende, Grazielle Cossenzo Florentino Galinari, Thiago L.M. Arcebispo |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Indirect elisa Diagnostic methods diagnosis Concordance Veterinary medicine 030106 microbiology Virus 03 medical and health sciences SF600-1100 Medicine General Veterinary Bovine leukemia virus biology business.industry iELISA bovine biology.organism_classification Ouchterlony double immunodiffusion Virology Enzootic Bovine Leukosis 030104 developmental biology cattle Enzootic bovine leukosis Enzootic enzyme-linked immunosorbent assay business control |
| Zdroj: | Pesquisa Veterinária Brasileira v.40 n.12 2020 Pesquisa Veterinária Brasileira Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA Pesquisa Veterinária Brasileira, Vol 40, Iss 12, Pp 977-984 (2021) |
| Popis: | Enzootic bovine leukosis (EBL) is an infectious disease caused by bovine leukemia virus (BLV) that affects cattle worldwide. Agar gel immunodiffusion (AGID) was the reference test for EBL diagnosis for many years, but enzyme-linked immunosorbent assay (ELISA) showed higher sensitivity, was faster to perform, and resulted in an objective reading. However, the importation of ELISA kits is lengthy and expensive, and currently, no AGID kits are available in Brazil. The aim of this work was to standardize an indirect ELISA (iELISA) for EBL diagnosis using BLV antigens produced in Tadarida brasiliensis lung (Tb1Lu) cells, which are Bovine viral diarrhea virus (BVDV) free, unlike fetal lamb kidney (FLK) cells, currently used for this purpose. Following standardization, iELISA results were compared with those obtained by AGID and the commercial Chekit Leucose-Serum ELISA. Compared to AGID, iELISA had 94,44% sensitivity, 75.68% specificity, 79.10% positive predictive value (PPV) and 93.30% negative predictive value (NPV), with 84% concordance and a Kappa index of 0.699. Compared to the Chekit Leucose-Serum ELISA, iELISA showed 92.60% sensitivity, 87.09% specificity, 90.27% PPV and 90,00% NPV, with 90.27% concordance and a Kappa index of 0.801. Taking into account the high agreement with the traditional tests and the absence of non-specific reactions with BVDV, the developed assay could be used as diagnostic method to control EBL in Brazil. |
| Databáze: | OpenAIRE |
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